DABSYL Amidite (T Linker Arm)
货号：BNS-5061-100,BNS-5061-250,BNS-5061-1
规格：100 µmol,250 mg,1 g
价格：4572.75,9497.25,30954
产品类型：核酸合成
品牌：LGC Biosearch Technologies
| Dabsyl is a nonfluorescent amidite that quenches in the green region of the visible spectrum. This amidite contains a DMT protecting group and can be added internally to the oligonucleotide. |
| 特性： |
| - Chemical Name:5’(4,4’dimethoxytrityl)-5-[N(6-4’sulfonyl-4-(dimethylamino)azobenzene)aminohexyl)-3-acrylamido]-2’-deoxyuridine-3’-(2-cyanoethyl)-(N,N’-Diisopropyl)-phosphoramidite- Formula:C62H76N9O11PS- Molecular Weight:1186.36- Appearance:orange solid- Absorption Maximum (Lambda Max):482- Extinction Coefficient at Lambda max:25500- Extinction Coefficient at 260 nm:20000- Fluorescence Maximum:480 |
| 产品用途： |
| Synthesis conditions:Prior to dilution ensure that all product is at the bottom of the vial. Dilute to the recommended concentration and mix thoroughly in the sealed vial to ensure that all contents are dissolved.Dilution:100 µmol/mLCoupling:10-minute couplingDeprotection conditions:Deprotection conditions are dependent upon the type of amidites used for the synthesis of the oligo. If using fast deprotecting amidites deprotect in concentrated NH4OH for 1 hour at 60 °C. If using standard amidites deprotect in concentrated NH4OH for 5 hours at 60 °C.Image of cleaved and deprotected structure: The mass this product adds after conjugation and work-up (the additional mass seen by mass spectrometry) is:745.74 |
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