Mouse IgA Isotype Control (S107), eBioscience™
货号： 14-4762-37,14-4762-81
规格：2 mg,50 µg
价格：11967,721
产品类型：小鼠同型对照
品牌：eBioscience
物种：小鼠
宿主：小鼠
抗体亚型：IgA
克隆号：S107
荧光染料：Purified
| 抗体类型: | 一抗 | 同型对照： | |
| 浓度： | 0.5 mg/mL | 用法： | Assay-Dependent/test(Flow) |
产品详细信息
Description: The mouse IgA kappa is useful as an isotype control immunoglobulin.Applications Reported: Purified Mouse IgA Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: The mouse IgA, kappa, immunoglobµLin has been tested as an isotype-matched control for immunofluorescence staining with flow cytometric analysis of normal human peripheral blood cells. Use isotype control at the same concentration as experimental antibody.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.仅用于科研。不用于诊断过程。
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| 参考文献： |
| 1.The Journal of experimental medicineIgA regulates the composition and metabolic function of gut microbiota by promoting symbiosis between bacteria."14-4762 was used in an ELISA assay to reveal a novel mechanism by which IgA promotes symbiosis and colonic homeostasis."AuthorsNakajima A,Vogelzang A,Maruya M,Miyajima M,Murata M,Son A,Kuwahara T,Tsuruyama T,Yamada S,Matsuura M,Nakase H,Peterson DA,Fagarasan S,Suzuki K |
QQ:1749072012