FITC Mouse anti-Human TRA-1-60

货号：NW-560876

规格：25 tests

价格：0

产品类型：流式抗体

品牌：BD

抗原：human TRA-1-60

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：TRA-1-60

荧光染料：FITC

Name:	FITC Mouse anti-human Tra-1-60		Catalog #:	NW-560876
Antigen:	Tra-1-60		Clone:	Tra-1-60
Host:	Mouse		Isotype :	IgM
Species:	Human		Conjugate :	FITC
Class:	Monoclonal		Size :	25 tests
Applications :	FC

The TRA-1-60 monoclonal antibody reacts with the neuraminidase-resistant form of a pluripotent-stem-cell-specific epitope on a high-molecular-weight transmembrane glycoprotein. The TRA-1-60 antigen is a sialylated epitope on the same keratan sulfate core molecule, podocalyxin, as 4 other distinct antigens on tumor-derived cell lines, TRA-1-81, GCTM2, K4, and K21. The expression of TRA-1-60 antigen is stage-specific and can be used to acterize embryonic cells and monitor their differentiation. The antigen is found on teratocarcinoma (embryonal carcinoma or EC), embryonic inner cell mass (but not morula or trophoblast), and embryonic stem (ES) cells. TRA-1-60 antigen is released into the serum of patients bearingtesticular tumors containing EC cells. As human EC and ES cells undergo differentiation, expression of TRA-1-60 antigen is lost.Expression of TRA-1-60 antigen has also been observed on a rhesus monkey ES cell line (Thomson et al, 1995).FITC, fluorescein isothiocyanate, is a fluorochrome with a molecular weight of 389 Da. FITC is sensitive to pH changes and photobleaching. Due to nearly identical excitation and emission properties but different spillover characteristics, FITC and Alexa Fluor® 488 cannot be used simultaneously. FITC is relatively dim and should be reserved for highly expressed markers whenever possible.

Fomat

Format FITCExcitation Source Blue 488 nmExcitation Max 494 nmEmission Max 520 nm

技术参数

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