IM-12
货号：04-0081
规格：2 mg
价格：0
产品类型：小分子化合物
品牌：Stemgent
The indolylmaleimide IM-12 is a potent enzymatic (IC50= 52 nM, compared with 92 nM for SB-216763) and intracellular inhibitor of GSK-3β which activates downstream components of canonical Wnt signaling and increasing β-catenin levels. IM-12 promotes increased differentiation of neural progenitor cells to neurons1. IM-12 has been shown to support the conversion and maintenance of human naïve stem cells2.
Stemgent conveniently providesallthe componentscomprisingthe 5i/L/A media supplment necessary to support naive pluripotent stem cell applications.
5i(inhibitors):
L:LIF, Human Recombinant(03-0016), and
A:Activin A, Human Recombinant(03-0001)
产品详情
| Product Name | StemoleculetmIM-12 |
| Calalog Number | 04-0081 |
| Size | 2 mg |
| Alternate Name | 3-(4-Fluorophenylethylamino)-1-methyl-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione |
| Chemical Formula | C22H20FN3O2 |
| Molecular Weight | 377.41 |
| CAS Number | 1129669-05-1 |
| Purity | 99% Purity by HPLC analysis |
| Appearance | Orange solid |
| Solubility | IM-12 is soluble in DMSO at 100 mM and in ethanol at 10 mM at room temperature. For a 10 mM concentrated stock solution of IM-12, reconstitute the compound by adding 530 μL of DMSO to the entire contents of the vial. If precipitate is observed, warm the solution to 37○C for 2 to 5 minutes.The maximum concentration recommended for dilution in media to ensure complete solubility is 10 μMFor cell culture, the media should be pre-warmed prior to adding the reconstituted compountd. Note: for most cells, the maximum tolerance to DMSO is less than 0.5% by volume. |
| Storage and Stability | Store powder for up to six months at 4○C, protected from light. Following reconstitution, store aliquots at -20○C. |
| Quality Control | The purity of IM-12 was determined by HPLC analysis. The accurate mass was determined by mass spectrometry.Cellular toxicity of IM-12 was tested on mouse embryonic stem cells. |
参考文献
Theunissen TW, Powell BE, Wang H, Mitalipova M, Faddah DA, Reddy J, Fan ZP, Maetzel D, Ganz K, Shi L, Lungjangwa T, Imsoonthornruksa S, Stelzer Y, Rangarajan S, D’Alessio A, Zhang J, Gao Q, Dawlaty MM, Young RA, Gray NS, Jaenisch R. “Systematic identification of culture conditions for induction and maintenance of naïve human pluripotency.” Cell Stem Cell 15(4):471-87 (2014).
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