Mouse anti Human CD33:StarBright Violet 790
货号：MCA1271SBV790
规格：100 Tests/0.5ml
价格：4276
产品类型：流式抗体
品牌：Bio-Rad
抗原：CD33
物种：人
宿主：小鼠
抗体亚型：IgG1
克隆号：WM53
荧光染料：StarBright Violet 790
| 产品介绍 | | |
| Mouse anti Human CD33 antibody, clone WM53recognizes the human CD33 cell surface glycoprotein. This antigen, considered to be specific for the myeloid lineage, has also been reported to be present on cells of lymphoid origin.Mouse anti Human CD33 antibody, clone WM53 immunoprecipitates a protein of ~75 kDa from myeloid cells, a smaller protein of approximately 67 kDa has been observed in immunoprecipitates from lymphoid targets. |
| 产品详情 | | |
Target Species | Human |
| Product Form | Purified IgG conjugated to StarBright Violet790 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3)1% Bovine Serum Albumin0.1% Pluronic F680.1% PEG 33500.05% Tween 20 |
| Immunogen | Human AML cells |
Fusion Partners | Spleen cells from immunized BALB/c mice were fused with cells of the mouse NSI myeloma cell line |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
StarBright Violet 790 | 401 | 782 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4°C. DO NOT FREEZE.This product should be stored undiluted. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 5ul of the suggested working dilution to label 106 cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application. |
| 数据 |
| Figure A. FITC conjugated Mouse anti Human CD14 (MCA1568F). Figure B. FITC conjugated Mouse anti Human CD14 (MCA1568F) and StarBright Violet 790 conjugated Mouse anti Human CD33 (MCA1271SBV790). All experiments performed on red blood cell lysed human blood gated on live single mononuclear cells, in the presence of 10% human serum. Data acquired on the ZE5 Cell Analyzer. |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Human Seroblock | BUF070A | F | 50 Test |
| Human Seroblock | BUF070B | F | 200 Test |
| 文献 |
| 1. Lajaunias, F.et al.(2005) Constitutive repressor activity of CD33 on human monocytes requires sialic acid recognition and phosphoinositide 3-kinase-mediated intracellular signaling.Eur J Immunol. 35: 243-51.2. Pietschmann, P.et al.(2000) Surface markers and transendothelial migration of dendritic cells from elderly subjects.Exp Gerontol. 35: 213-24.3. Favaloro, E.J.et al. (1993) Differential expression of surface antigens on activated endothelium.Immunol Cell Biol. 71:571-81.4. Yasukawa, T.et al.(2012) Simple detection of surface antigens on living cells by applying distinct cell positioning with negative dielectrophoresis.Anal Chem. 84 (20): 8830-6.5. Hu, Z.et al.(2016) Self-assembled nanoparticles based on folic acid modified carboxymethyl chitosan conjugated with targeting antibodyJ Wuhan Univ of Technol-Mater. Sci. Ed. 31 (2): 446-53.6. Dahl Cet al.(2004) Human mast cells express receptors for IL-3, IL-5 and GM-CSF; a partial map of receptors on human mast cells culturedin vitro.Allergy. 59 (10): 1087-96.7. Vamvakopoulos, J.E. & Green, C. (2003) HMG-CoA reductase inhibition aborts functional differentiation and triggers apoptosis in cultured primary human monocytes: a potential mechanism of statin-mediated vasculoprotection.BMC Cardiovasc Disord. 3: 6.8. Vamvakopoulos, J.et al.(2002) Genetic control of IL-1beta bioactivity through differential regulation of the IL-1 receptor antagonist.Eur J Immunol. 32 (10): 2988-96.9. Lin, C.W.et al.(2005) CD94 1A transcripts characterize lymphoblastic lymphoma/leukemia of immature natural killer cell origin with distinct clinical features.Blood. 106 (10): 3567-74.10. McCormack Eet al.(2013) Multiplexed mAbs: a new strategy in preclinical time-domain imaging of acute myeloid leukemia.Blood. 121 (7): e34-42. |
技术参数 产品应用 Flow
产品类型 Monoclonal Antibody
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