| 产品介绍 | | |
| Rat anti Mouse CD117 antibody, clone 2B8recognizes mouse CD117, a ~150 kDa cell surface molecule otherwise known as the Kit, or c-kit, receptor.Rat anti Mouse CD117 antibody, clone 2B8 recognizes an epitope within the extracellular region of CD117. In mice,CD117 is predominantly expressed on multipotent haematopoietic stem cells from the adult bone marrow and foetal liver. |
| 产品详情 | | |
Target Species | Mouse |
| Product Form | Purified IgG conjugated to StarBright UltraViolet 400 - liquid |
Preparation | Purified IgG prepared by affinity chromatography on Protein G from tissue culture supernatant |
BufferSolution | Phosphate buffered saline |
Preservative Stabilisers | 0.09% Sodium Azide (NaN3) 1% Bovine Serum Albumin 0.1% Pluronic F68 0.1% PEG 3350 0.05% Tween 20 |
| Immunogen | IL-3 dependent mast cells. |
| Approx. Protein Concentrations | IgG concentration 0.05 mg/ml |
Fusion Partners | Spleen cells from immunised Wistar rats were fused with cells of the Fox-NY mouse myeloma cell line. |
Max Ex/Em | Fluorophore | Excitation Max (nm) | Emission Max (nm) |
StarBright UltraViolet 400 | 347 | 394 |
Regulatory | For research purposes only |
Guarantee | 12 monthsfrom date of despatch |
| 存储条件 | | |
| Store at +4°C. DO NOT FREEZE.This product should be stored undiluted. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
Flow Cytometry | √ | Neat | Neat |
| This product has been reported to work in the following applications. This information is derived from testing within our laboratories, peer-reviewed publications or personal communications fromthe originators. Please refer to references indicated for further information. For general protocol recommendations, please visit theantibody protocolspage.Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls.Use 5ul of the suggested working dilution to label 106cells in 100ul. Best practices suggest a 5 minutes centrifugation at 6,000g prior to sample application. |
| 相关产品 |
| Description | Product Code | Applications | Pack Size |
| Mouse Seroblock FcR | BUF041A | F | 0.1 mg |
| Mouse Seroblock FcR | BUF041B | F | 0.5 mg |
| 数据 |
| Figure A. StarBright Violet 670 conjugated Rat anti Mouse CD11b (MCA711SBV670). Figure B. StarBright Violet 670 conjugated Rat anti Mouse CD11b (MCA711SBV670) and StarBright UltraViolet 400 conjugated Rat anti Human CD117 (MCA1365SBUV400). All experiments performed on murine bone marrow gated on live single cells, in the presence of 10% mouse serum. Data acquired on the ZE5 Cell Analyzer. |
| 文献 |
| 1. Fleming WHet al.(1993) Steel factor influences the distribution and activity of murine hematopoietic stem cellsin vivo.Proc Natl Acad Sci U S A. 90 (8): 3760-4.2. Rico-Vargas, S.A.et al.(1994) c-kit expression by B cell precursors in mouse bone marrow. Stimulation of B cell genesis by in vivo treatment with anti-c-kit antibody.J Immunol. 152 (6): 2845-52.3. Manova, K.et al.(1992) c-kit receptor and ligand expression in postnatal development of the mouse cerebellum suggests a function for c-kit in inhibitory interneurons.J Neurosci. 12 (12): 4663-76.4. Maeda, H.et al.(1992) Requirement of c-kit for development of intestinal pacemaker system.Development. 116 (2): 369-75.5. Takagi, M.et al.(1992) Stimulation of mouse connective tissue-type mast cells by hemopoietic stem cell factor, a ligand for the c-kit receptor.J Immunol. 148 (11): 3446-53.6. de Vries, P.et al.(1992) Thymus reconstitution by c-kit-expressing hematopoietic stem cells purified from adult mouse bone marrow.J Exp Med. 176 (6): 1503-9. |
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