Anti-Mouse TCR beta PerCP-Cy5.5

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Anti-Mouse TCR beta PerCP-Cy5.5

货号:89612-70-25,89612-70-100

规格:25 μg,100 μg

价格:880,2279

产品类型:流式抗体

品牌:Biogems

物种:人

宿主:小鼠

抗体亚型:IgG

荧光染料:Purified

Catalogue Number :89612-70

Description

The H57-597 monoclonal antibody is specific for the beta chain of the mouse T cell Receptor (TCR). The crosslinking induces activation and proliferation of T cells, as a plate-bound or a soluble H57-597, or the plate-bound antibody can induce apoptosis, based on assay conditions. The beta chain of the TCR can combine with the alpha chain of the receptor to produce the alpha-beta TCR, which is expressed by the NKT cells, by the NK1.1+ thymocytes and most of the T cells. The beta chain does not react with the gamma-delta TCR-bearing cells, expressed by a small number of T cells.

This antibody can be used as a phenotypic marker for the TCR beta expressing cells or for the functional purpose of TCR-mediated cell activation or apoptosis.

C57Bl/6 splenocytes were stained with PerCP-Cy5.5 H57-597 with relevant isotype control in Red.

Additional Information

Clone

H57-597

Format:

PerCP-Cyanine5.5

Applications

FC

Reactivity

Mouse

Isotype:

Armenian Hamster IgG

Research Interest:Adaptive Immunity
Cell Type:T Cells

Preperation:

The product should be stored undiluted at 4°C and should be protected from prolonged exposure to light. Do not freeze. The monoclonal antibody was purified utilizing affinity chromatography and unreacted dye was removed from the product.

Formulation:

Phosphate-buffered aqueous solution, ≤0.09% Sodium azide, may contain carrier protein/stabilizer, ph7.2.

References:

Kubo, R. T., Born, W., Kappler, J. W., Marrack, P., & Pigeon, M. (1989). Characterization of a monoclonal antibody which detects all murine alpha beta T cell receptors.The Journal of Immunology,142(8), 2736-2742.

Gascoigne, N. R. (1990). Transport and secretion of truncated T cell receptor beta-chain occurs in the absence of association with CD3.Journal of Biological Chemistry,265(16), 9296-9301.

Kruisbeek, A. M., Shevach, E., & Thornton, A. M. (2004). Proliferative assays for T cell function.Current Protocols in Immunology, 3-12.

Saint-Ruf, C., Panigada, M., Azogui, O., Debey, P., von Boehmer, H., & Grassi, F. (2000). Different initiation of pre-TCR and γδTCR signalling.Nature,406(6795), 524-527.

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