HapCult™ Episomal iPSC Reprogramming Kit/iPS重编程试剂盒（CD34+细胞、血细胞重编程）

货号：611005,611010

规格：5 tests,10 tests

价格：9600,17000

产品类型：多能干细胞培养

品牌：PBM

Episomal iPSC Reprogramming Kit 是一种高效的非基因插入整合型重编程系统，可将体细胞重编程为诱导多能干细胞（Induced pluripotent stem cells, iPSCs）。其利用多能性因子Oct4、 Sox2、 Klf4、 c-Myc 的非整合型质粒载体诱导体细胞生成无外源基因插入整合的 iPSCs，对于重编程难度较大的悬浮细胞，此系统重编程效率较高，如外周血单个核细胞（Peripheralblood mononuclear cells, PBMCs）。由于 Episomal iPSC Reprogramming Kit 重编程效率高、实验取材方便（5—10ml 外周血)、操作简单，并且无外源基因插入整合等优点， 适合用于 iPSCs 疾病模型的建立、药物开发、细胞治疗以及其它再生医学研究领域。

数据
	←iPSC免疫荧光染色（TRA-1-60，SSEA4，NANOG，OCT4，DAPI） ↓注射免疫缺陷小鼠8周畸胎瘤(左 1)和三胚层 H.E.染色结果(左2，右1，右2 )
注意：人胚胎干细胞（hES）或诱导多能干细胞(hiPS)在无滋养层细胞培养条件下培养换液时，请将新鲜的培养液沿培养容器壁缓慢添加，避免液体的冲击力将贴壁的细胞吹起脱离培养皿。
参考文献
1. Meng X, Neises A, Su RJ, Payne KJ, Ritter L, Gridley DS, Wang J, Sheng M, Lau KH, Baylink DJ, Zhang XB: Efficient reprogramming of human cord blood CD34+ cells into induced pluripotent stem cells with OCT4 and SOX2 alone. Molecular Therapy, 2012;20(2):408-16.2. Su RJ, Baylink DJ, Neises A, Kiroyan JB, Meng X, Payne KJ, Tschudy-Seney B, Duan Y, Appleby N, Kearns-Jonker M, Gridley DS, Wang J, Lau KHW, Zhang XB: Efficient generation of integration-free iPS cells from human adult peripheral blood using BCL-XL together with Yamanaka factors. PLoS One. 2013, 8(5): e64496.3. Zhang XB: Cellular reprogramming of human peripheral blood cells. Genomics Proteomics Bioinformatics. 2013 Oct;11(5):264-74.4. Su RJ, Neises A, Zhang XB: Generation of iPS cells from human peripheral blood mononuclear cells using episomal vectors. Methods Mol Biol. 2016;1357:57-69.5. Wen W#, Zhang JP#, Xu J#, Su RJ, Neises A, Ji GZ, Yuan W, Cheng T*, Zhang XB*. Enhanced generation of integration-free iPSCs from human adult peripheral blood mononuclear cells with an optimal combination of episomal vectors. Stem Cell Reports. 2016 Jun 14;6(6):873-84.6. Wen W, Zhang JP, Chen W, Arakaki C, Li XL, Baylink D, Botimer GD, Xu J, Yuan W, Cheng T*, Zhang XB*. Generation of integration-free induced pluripotent stem cells from human peripheral blood mononuclear cells using episomal vectors. J Vis Exp. 2017 Jan 1;(119). doi: 10.3791/55091.
已发表文献
1.Zhang Haiyana,Ma Yanyana,Yu Shujuanb,et al.Establishment of a human iPSC line (SDQLCHi010-A) from a patient with optic nerve malformation carrying a heterozygous mutation in PAX6 gene.Stem Cell Research.Stem Cell Research 41 (2019) 101611

技术参数

产品优点 高效率无基因整合插入取材方便，5-15mL 血液即可重编程

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