CD152 (CTLA-4) Monoclonal Antibody (14D3), PE, eBioscience™

货号：12-1529-42,12-1529-41

规格：100 tests,25tests

价格：2623,1065

产品类型：流式抗体

品牌：eBioscience

抗原：CD152

物种：人/恒河猴

宿主：小鼠

抗体亚型：IgG2a, kappa

克隆号：14D3

荧光染料：PE

抗体类型:	流式抗体	同型对照：	Mouse IgG2a kappa Isotype Control (eBM2a), PE, eBioscience
浓度：	5 µL/Test	用法：	5 µL (0.03 µg)/test (Flow）

产品详细信息Description:The 14D3 monoclonal antibody reacts with human CD152, also known as cytotoxic T lymphocyte antigen-4 (CTLA-4). CTLA-4, a protein with structural similarities to CD28, is expressed on activated T cells (activated B cells may also express CTLA-4) and binds the B7 family members, CD80 (B7-1) and CD86 (B7-2), with higher affinity than CD28 does. CTLA-4 and CD28 appear to deliver opposing signals to T cells: while CD28 is a potent costimulator, CTLA-4 restricts the progression of T cellsto an activated state by inhibiting IL-2 secretion and cellular proliferation. The cytoplasmic portion of CTLA-4 contains ER retention motifs, resulting in intracellular localization of a large proportion of newly synthesized CTLA-4 in response to TCR signaling.The 14D3 antibody also recognizes rhesus monkey and has inhibitor activity.Applications Reported: The 14D3 antibody has been reported for use in flow cytometric analysis.Applications Tested: This 14D3 antibody has been pre-titrated and tested by intracellular staining and flow cytometric analysis of stimulated human peripheral blood cells using the Intracellular Fixation and Permeabilization Buffer Set (cat. 88-8824) and protocol. Please refer to Best Protocols: Protocol A: Two step protocol for (cytoplasmic) intracellular proteins located under the Resources Tab online. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Furthermore, due to the intracellular localization of a large portion of CTLA-4, for complete detection it may be necessary to assess intracellular expression, in addition to surface expression of CTLA-4.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationCTLA4 (Cytotoxic T-Lymphocyte Antigen 4) is a CD28-family receptor expressed on mainly CD4+ T cells. It binds the same ligands as CD28 (CD80 and CD86 on B cells and dendritic cells), but with higher affinity than CD28. However, in contrast to CD28 which enhances cell function when bound at the same time as the T cell receptor, CTLA4 inhbits the T cell and prevents it from functioning. Intracellular CTLA4 is also found in regulatory T cells and may be important to their function.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD152 (CTLA-4) Antibody (12-1529-41) in FlowPHA-stimulated human peripheral blood cells were intracellularly stained with Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (blue histogram) or Anti-Human CD152 (CTLA-4) PE (purple histogram) using the Intracellular Fixation and Permeabilization Buffer Set (Product # 88-8824-00) and protocol. Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.JCI insightMitochondrial dysregulation and glycolytic insufficiency functionally impair CD8 T cells infiltrating human renal cell carcinoma."12-1529 was used in Flow cytometry/Cell sorting to show that improving metabolic function of clear cell renal cell carcinoma CD8 tumor infiltrating lymphocytes may promote the immune response."2.OncoimmunologyAzacytidine prevents experimental xenogeneic graft-versus-host disease without abrogating graft-versus-leukemia effects."12-1529 was used in Flow cytometry/Cell sorting to investigate the impact of 5-azacytidine on xenogeneic graft-vs.-host disease and graft-vs.-leukaemia effects in a humanised murine model of transplantation."3.Frontiers in immunologySchistosoma mansonirSm29 Antigen Induces a Regulatory Phenotype on Dendritic Cells and Lymphocytes From Patients With Cutaneous Leishmaniasis."12-1529 was used in Flow cytometry/Cell sorting to evaluate the potential of the S.mansoni Sm29 antigen to change aspects of profiles of monocyte derived dendritic cells and lymphocytes from subjects with cutaneous leishmaniasis."4.International journal of nanomedicineFunctionalization-dependent effects of cellulose nanofibrils on tolerogenic mechanisms of human dendritic cells."12-1529 was used in Flow cytometry/Cell sorting to investigate how differentially functionalised cellulose nanofibrils affect dendritic cell properties, their viability, morphology, differentiation and maturation potential, and the capacity to regulate T cell-mediated immune response."

技术参数

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