Rat IgG2a kappa Isotype Control, PE, eBioscience™

货号：12-4321-80,12-4321-81,12-4321-82,12-4321-83

规格：25 ug,50 ug,100 ug,200 ug

价格：750,1169,1702,2448

产品类型：大鼠同型对照

品牌：eBioscience

物种：其它

宿主：大鼠

抗体亚型：IgG2a, kappa

荧光染料：PE

类型:	同型对照	同型对照：
浓度：	0.2 mg/mL	用法：	Assay-Dependent(Flow)；Assay-Dependent(Ctrl)产品详细信息

Description: The monoclonal rat IgG2a, kappa is useful as an isotype control immunoglobulin.Applications Reported: This rat IgG2a isotype control has been reported for use in flow cytometric analysis.Applications Tested: This Rat IgG2a K Isotype Control has been tested by flow cytometric analysis of mouse splenocytes and should be used at the same concentration as the experimental antibody.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息The isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.

数据

Rat IgG2a kappa Isotype Control (12-4321-80) in ICCNormal human peripheral blood cells were cultured for 3 hr with dexamethasone, followed by intracellular staining with 5 µg/mL of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (inset) or 5 µg/mL of Anti-Human/Mouse GILZ PE. Cells were cytospun onto glass slides and the nuclei were counterstained with DAPI.

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参考文献

1. OncoimmunologyMetronomic cyclophosphamide eradicates large implanted GL261 gliomas by activating antitumor Cd8+T-cell responses and immune memory.2. VirologyRole of regulatory T cells in coronavirus-induced acute encephalitis.3. PloS oneA chitin-like component on sclerotic cells of Fonsecaea pedrosoi inhibits Dectin-1-mediated murine Th17 development by masking β-glucans.4. MethodsXA method for conducting suppression assays using small numbers of tissue-isolated regulatory T cells.5. PloS oneIn Vitro Influence of Mycophenolic Acid on Selected Parameters of Stimulated Peripheral Canine Lymphocytes.

技术参数

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