NK1.1 Monoclonal Antibody (PK136), PE, eBioscience™
货号：12-5941-82
规格：100 UG
价格：1886
产品类型：流式抗体
品牌：eBioscience
物种：小鼠
宿主：小鼠
抗体亚型：IgG
荧光染料：PE
| Tested Species reactivity | Mouse |
| Published species reactivity | Mouse |
| Host / Isotype | Mouse / IgG2a, kappa |
| Recommended Isotype Control | Mouse IgG2a kappa Isotype Control, PE, eBioscience™ |
| Class | Monoclonal |
| Type | Antibody |
| Clone | PK136 |
| Conjugate | PE |
| Form | Liquid |
| Concentration | 0.2 mg/mL |
| Purification | Affinity chromatography |
| Storage buffer | PBS, pH 7.2, with 0.1% gelatin |
| Contains | 0.09% sodium azide |
| Storage conditions | 4° C, store in dark, DO NOT FREEZE! |
| RRID | AB_466050 |
Product Specific Information
Description: The PK136 monoclonal antibody reacts with mouse NK1.1, an antigen expressed by natural killer cells and a subset of T cells in the NK1.1 mouse strains including C57BL and NZB. Several commonly used laboratory mouse strains such as BALB/c, SJL, AKR, CBA, C3H and A do not express the NK1.1 antigen. For detection of NK cells in these strains the monoclonal antibody DX5 (Cat. No. 14-5971) should be used. Simultaneous staining of C57BL/6 spleen cells with PK136 and DX5 reveals coexpression of both markers by a majority of cells as well as presence of small populations of DX5+PK136- and DX5-PK136+ cells.Applications Reported: PK136 has been reported for use in flow cytometric analysis.Applications Tested: The PK136 antibody has been tested by flow cytometric analysis of C57Bl/6 mouse splenocytes and can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.
Background/Target Information
Killer cell lectin-like receptor subfamily B, member 1, also known as KLRB1, NKR-P1A or CD161 (cluster of differentiation 161), is a human gene. Natural killer (NK) cells are lymphocytes that mediate cytotoxicity and secrete cytokines after immune stimulation. Several genes of the C-type lectin superfamily, including the rodent NKRP1 family of glycoproteins, are expressed by NK cells and may be involved in the regulation of NK cell function. The KLRB1 protein contains an extracellular domain with several motifs characteristic of C-type lectins, a transmembrane domain, and a cytoplasmic domain. The KLRB1 protein, NKR-P1A or CD161, is classified as a type II membrane protein because it has an external C terminus. NKR-P1A, the receptor encoded by the KLRB1 gene, recognizes Lectin Like Transcript-1 (LLT1) as a functional ligand.
For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
Excitation/Emission Profile
数据
Staining of C57Bl/6 splenocytes with Anti-Mouse CD49b (Integrin alpha 2) eFluor® 450 (Product # 48-5971-82) and 0.125 µg of Mouse IgG2a K Isotype Control PE (Product # 12-4724-81) (left) or 0.125 µg of Anti-Mouse NK1-1 PE (right). Cells in the lymphocyte gate were used for analysis.
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