anti-mouse CD19 (EBIO1D3) PE
货号：12-0193-82,12-0193-83,12-0193-81,12-0193-85
规格：100ug,200ug,50ug,500ug
价格：1880,3037,1062,5772
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号： eBio1D3 (1D3)
荧光染料：PE
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow；MISC；FN；FI |
| 推荐用法： | 0.125 µg/test（flow) |
| Description: The eBio1D3 monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 数据 |
CD19 Antibody (12-0193-82) Staining of BALB/c splenocytes with Anti-Human/Mouse CD45R (B220) APC (Product # 17-0452-82) and 0.06 µg of Rat IgG2a kappa Isotype Control PE (Product # 12-4321-80) (left) or 0.06 µg of Anti-Mouse CD19 PE (right). Total viable cells were used for analysis. |
| 推荐产品： |
| Flow Cytometry Staining Buffer, eBioscienceUltracomp eBeads microspheresLIVE/DEAD® Fixable Cell Stains |
| 参考文献： |
| 1. PloS one Alkylator-Induced and Patient-Derived Xenograft Mouse Models of Therapy-Related Myeloid Neoplasms Model Clinical Disease and Suggest the Presence of Multiple Cell Subpopulations with Leukemia Stem Cell Activity. "12-0193 was used in Flow cytometry/Cell sorting to report several new t-AML/MDS mouse models that could potentially be used to further define disease pathogenesis and test novel therapeutics." Authors Jonas BA,Johnson C,Gratzinger D,Majeti R2. Nature cell biology Medial HOXA genes demarcate haematopoietic stem cell fate during human development. View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3. Scientific reports Protein Kinase D2 Protects against Acute Colitis Induced by Dextran Sulfate Sodium in Mice. View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer4. PloS one Favorable alteration of tumor microenvironment by immunomodulatory cytokines for efficient T-cell therapy in solid tumors. "12-0193 was used in Flow cytometry/Cell sorting to alter the tumor microenvironment using immunomodulatory cytokines for efficient T-cell therapy in solid tumors." Authors Tähtinen S,Kaikkonen S,Merisalo-Soikkeli M,Grönberg-Vähä-Koskela S,Kanerva A,Parviainen S,Vähä-Koskela M,Hemminki A5. Journal of immunology (Baltimore, Md. : 1950) IL-10+ Innate-like B Cells Are Part of the Skin Immune System and Require α4β1 Integrin To Migrate between the Peritoneum and Inflamed Skin. "12-0193 was used in Flow cytometry/Cell sorting to investigate innate-like B cells within the skin." Authors Geherin SA,Gómez D,Glabman RA,Ruthel G,Hamann A,Debes GF |
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