货号：110707

规格：50 µg

价格：910

产品类型：流式抗体

品牌：Biolegend

物种：小鼠

宿主：仓鼠

抗体亚型：IgG

荧光染料：PE

Description

CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (e.g., RIII, SJL/J, STS/A, DA). CD45, a member of the protein tyrosine phosphatase (PTP) family, is a 180-240 kD glycoprotein expressed on all hematopoietic cells except mature erythrocytes and platelets. There are multiple isoforms in mice that play key roles in TCR and BCR signal transduction. These isoforms are very specific to the activation and maturation states of the cell as well as specific cell types. The primary ligands for CD45 are galectin-1, CD2, CD3, CD4, TCR, CD22, and Thy-1.

• Isotype Control

• PE Mouse IgG2a, κ Isotype Ctrl

• Reactivity

• Mouse

• Immunogen

• SJL mouse thymocytes and splenocytes

• Formulation

• Phosphate-buffered solution, pH 7.2, containing 0.09% sodium azide.

• Preparation

• The antibody was purified by affinity chromatography, and conjugated with PE under optimal conditions. The solution is free of unconjugated PE and unconjugated antibody.

• Concentration

• 0.2 mg/ml

• Storage & Handling

• The CD45.1 antibody solution should be stored undiluted between 2°C and 8°C, and protected from prolonged exposure to light.Do not freeze.

• Application

• FC - Quality tested

• Recommended Usage

• Each lot of this antibody is quality control tested byimmunofluorescent staining with flow cytometric analysis. For flow cytometric staining, the suggested use of this reagent is ≤ 0.25 µg per 106cells in 100 µl volume. It is recommended that the reagent be titrated for optimal performance for each application.

• Excitation Laser

• Blue Laser (488 nm)

• Application Notes

• The A20 antibody does not react with leukocytes ormouse cells expressing the CD45.2 alloantigen. Additional reported applications (for relevant formats of this clone) include: immunoprecipitation3,in vitroblocking of B cell responses1,2, immunohistochemical staining of frozen sections: OCT embedded7and acetone-fixed4-6(direct immunofluorescence detection with fluorochrome conjugated A20 was used in (5) and (6)), and immunofluorescence microscopy9.

• Application References(PubMed link indicates BioLegend citation)

• 1. Yakura H,et al.1983.J. Exp. Med.157:1077. (Block)2. Yakura H,et al.1986.J. Immunol.136:2729. (Block)3. Shen FW,et al.1986.Immunogenetics24:146. (IP)4. Suzuki K,et al.2000.Immunity13:691. (IHC)5. Werner N,et al.2002.Arterioscler. Thromb. Vasc. Biol.22:1567. (IHC)6. Lessner SM,et al.2002.Am. J. Pathol.160:2145. (FC, IHC)7. Chen CC,et al.2005.P. Natl. Acad. Sci. USA102:11408 (IHC)8. Pascal V,et al.2007.J. Immunol.179:1751. (FC)9. Mende I,et al.2006.Blood107:1383. (IF, IHC, FC)10. Phan TG,et al.2007.Nature Immunol.8:992. (FC)11. Wither DR,et al.2009.J. Immunol.183:5079.PubMed12. Pascal V,et al.2007.J. Immunol.179:1751.PubMed13. Lee SW,et al.2009.J. Immunol.182:6753.PubMed14. Takada K,et al.2009.J. Exp Med.206:2253.PubMed15. Beamer CA,et al.2007.Am. J. Respir. Cell. Mol. Biol.37:729. (FC)PubMed16. Li LX,et al.2010.J. Immunol.184:1728.PubMed17. Hosoi A,et al.2008.Cancer Res.68:3941. (FC)PubMed18. Kenna TJ,et al.2008.Blood111:2091.PubMed19. Kohlmeier JE,et al.2008.Immunity.29:101. (FC)PubMed20. Leon-Rico D,et al.2015.Lab Anim.49:132.PubMed