货号：D4200

规格：50 Preps

价格：2410

产品类型：质粒

品牌：Zymo

ZymoPURE^™Plasmid Isolation kits provide the fastest and simplest method available to efficiently isolate transfection quality plasmid DNA fromE. coli.TheZymoPURE^™Plasmid Midiprepkit features a spin column-based method for the purification of up to 300 µg of high-quality plasmid DNA in less than 18 minutes. The eluted plasmid DNA is ready for immediate use, avoiding the need for subsequent precipitation steps.

ZymoPURE^™technology uses a modified alkaline lysis method and features novel binding chemistry that yields highly concentrated plasmid DNA (up to 3 µg/µl) directly from a spin column. The wash regimen has been optimized to ensure the plasmid DNA is free of endotoxins, salt, protein, and RNA resulting in plasmid DNA suitable for sensitive applications including transfection, plasmid-mediated gene silencing, cloning, and sequencing.

As an added convenience, the ZymoPURE^™Plasmid Midiprep contains colored buffers that permit error-free visualization and identification of complete bacterial cell lysis and neutralization. Syringe filters are included for rapid clearing of the lysate and the unique spin-column design allows the binding step to be performed using a vacuum or table top centrifuge.

- The fastest, easiest, most reliable method for purification of up to 300 µg of ultra-pure endotoxin-free plasmid DNA.

- Routinely recover ≥ 1 µg/µl plasmid DNA directly from a microcentrifuge column that is ideal for transfection and other sensitive downstream applications.

Plasmid DNA yield and concentration from the ZymoPure^™Maxiprep kit compared to other major suppliers. Plasmid DNA (pGEM^®) was isolated from 150 ml of JM109E. coliculture grown overnight following the manufacturer’s suggested protocol (in duplicate). One (1) µl of eluted plasmid DNA was visualized post agarose gel electrophoresis. M, ZR 1 kb DNA Marker (Zymo Research).