Quick-RNA™ MicroPrep
货号:R1051
规格:200preps
价格:7830
产品类型:RNA提取
品牌:Zymo
TheQuick-RNA™MicroPrepkit is an innovative product designed for the easy, reliable, and rapid isolation of DNA-free RNA from a wide range of cell(up to 106)and tissue samples(up to 5 mg). The procedure combines a unique buffer system withFast-Spincolumn technology to yield high quality total RNA(including small RNAs 17-200 nt)in about 10 minutes.
The procedure is simple: Add the providedRNA Lysis Bufferto a sample, then purify the RNA using theZymo-Spin™Columns. The result is highly-concentrated,DNA-freeRNA that is suitable for subsequent RNA-based methods including RT-PCR, hybridization, sequencingetc. In addition, the kit can be used for enrichment of small and large RNAs in two separate fractions.
产品特点
High-quality total RNA (including small RNAs) from a wide range of samples – single to 106cells.
Isolate small and large RNAs into separate fractions (optional).
DNA-free RNA for use in any downstream application. DNase I included.
组成成份
| Product | Size | Catalog # | How to Buy |
|---|
| RNA Lysis Buffer (100 ml) | 100 ml | R1060-1-100 | See Distributor |
| RNA Wash Buffer (48 ml) | 48 ml | R1003-3-48 | See Distributor |
| DNase/Rnase-Free Water | 10 ml | W1001-10 | See Distributor |
| RNA Prep Buffer (100 ml) | 100 ml | R1060-2-100 | See Distributor |
| DNase I Set | 1 Set | E1010 | See Distributor |
| Zymo-Spin™ IC | 50 Pack | C1004-50 | See Distributor |
| Collection Tubes (2 ml) | 50 Pack | C1001-50 | See Distributor |
数据资料
TheQuick-RNA™ MicroPrepyields high quality total RNA. High levels of genomic DNA contamination are present in the preps from Suppliers Q & P but not with theQuick-RNA™ MicroPrep. Total RNA was isolated from human epithelial cells (sans DNase treatment).
RNA isolated with theQuick-RNA™ MicroPrepis DNA-free. Samples isolated with Supplier Q's kit are provided for comparison. Total RNA was isolated from 106human epithelial cells (with in-column DNase treatments for both kits). Each amplification curve represents an average of three independent isolation experiments. |