Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5) eFluor 450

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Ly-6G/Ly-6C Monoclonal Antibody (RB6-8C5) eFluor 450

货号:48-5931-80,48-5931-82

规格:25μg,100μg

价格:1280,2385

产品类型:流式抗体

品牌:eBioscience

物种:小鼠

宿主:大鼠

抗体亚型:IgG2b, kappa

荧光染料:eFluor 450

抗体类型:单抗同型对照:IgG2b, kappa
免疫原性:Human embryonal carcinoma (EC) cell line 2102Ep用法:0.06 µg/test(flow)

Description: The RB6-8C5 monoclonal antibody reacts with mouse Ly-6G, a 21-25 kDa protein also known as the myeloid differentiation antigen Gr-1. A GPI-linked protein, Gr-1 is expressed by the myeloid lineage in a developmentally regulated manner in the bone marrow. While monocytes only express Gr-1 transiently during their bone marrow development, the expression of Gr-1 on bone marrow granulocytes as well as on peripheral neutrophils is a good marker for these populations.eBioscience testing indicates that in the bone marrow and lysed whole blood, the antibody clone RB6-8C5 also stains cells that express the highest levels of Ly6c (as defined by staining with antibody clone HK1.4). It is recommended that 1A8-Ly6G (cat. 9668) be used when looking at Ly-6G specific targets.Applications Reported: This RB6-8C5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RB6-8C5 antibody has been tested by flow cytometric analysis of mouse bone marrow cells. This can be used at less than or equal to 0.06 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered.
数据

Ly-6G (Gr-1) Antibody (48-5931-82)Staining of C57BL/6 bone marrow cells with Anti-Mouse CD11b FITC (Product # 11-0112-41) and 0.03 µg of Rat IgG2b kappa Isotype Control eFluor® 450 (Product # 48-4031-82) (left) or 0.03 µg of Anti-Mouse Ly-6G (Gr-1) eFluor® 450 (right). Total cells were used for analysis.

Ly-6G (Gr-1) Antibody (48-5931-82)

Staining of mouse splenocytes and bone marrow cells. As expected based on known relative expression patterns, Gr-1 clone RB6-8C5 stains cells in the bone marrow myeloid gate and not in the splenocytes gate or bone marrow lymphoid gate. Details: Balb/c bone marrow cells (left) and splenocytes (middle) were surface stained with Gr-1 (clone RB6-8C5) followed by staining with 7-AAD. Viable bone marrow cells in the lymphoid (blue histogram) and myeloid (purple histogram) gates and viable splenocytes (orange histogram) were used for analysis.

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参考文献:
1. Nature medicineJNK1 negatively controls antifungal innate immunity by suppressing CD23 expression."48-5931 was used in Flow cytometry/Cell sorting to investigate the mechanisms that negatively regulate host immune responses to a fungal infection."AuthorsZhao X,Guo Y,Jiang C,Chang Q,Zhang S,Luo T,Zhang B,Jia X,Hung MC,Dong C,Lin X

2. The Journal of experimental medicine

Eros is a novel transmembrane protein that controls the phagocyte respiratory burst and is essential for innate immunity."48-5931 was used in Flow cytometry/Cell sorting to describe the key role of the previously uncharacterised protein Eros in host defence."AuthorsThomas DC,Clare S,Sowerby JM,Pardo M,Juss JK,Goulding DA,van der Weyden L,Storisteanu D,Prakash A,Espéli M,Flint S,Lee JC,Hoenderdos K,Kane L,Harcourt K,Mukhopadhyay S,Umrania Y,Antrobus R,Nathan JA,Adams DJ,Bateman A,Choudhary JS,Lyons PA,Condliffe AM,Chilvers ER,Dougan G,Smith KG3. CellThe Spectrum and Regulatory Landscape of Intestinal Innate Lymphoid Cells Are Shaped by the Microbiome."48-5931 was used in Flow cytometry/Cell sorting to investigate the cellular states and regulatory landscapes of innate lymphoid cells."AuthorsGury-BenAri M,Thaiss CA,Serafini N,Winter DR,Giladi A,Lara-Astiaso D,Levy M,Salame TM,Weiner A,David E,Shapiro H,Dori-Bachash M,Pevsner-Fischer M,Lorenzo-Vivas E,Keren-Shaul H,Paul F,Harmelin A,Eberl G,Itzkovitz S,Tanay A,Di Santo JP,Elinav E,Amit I

3. Schopperle, W.M., and DeWolf, W.C. (2007) The TRA-1-60 and TRA-1-81 human pluripotent stem cell markers are expressed on podocalyxin in embryonal carcinoma. Stem Cells 25: 723-730.4. Chin, A.C., Fong, W.J., Goh, L.T., Philp, R., Oh, S.K., and Choo, A.B. (2007) Identification of proteins from feeder conditioned medium that support human embryonic stem cells. J Biotechnol. 130: 320-328.5. Badcock, G., Pigott, C., Goepel, J., and Andrews, P.W. (1999) The human embryonal carcinoma marker antigen TRA-1-60 is a sialylated keratan sulfate proteoglycan. Cancer Res. 59: 4715-4719.6. Andrews, P.W., Banting, G., Damjanov, I., Arnaud, D., and Avner, P. (1984) Three monoclonal antibodies defining distinct differentiation antigens associated with different high molecular weight polypeptides on the surface of human embryonal carcinoma cells. Hybridoma 3: 347-361.

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