anti-mouse CD19 (eBio1D3) SB702
货号：67-0193-82,67-0193-80
规格：100 ug,25 ug
价格：3293,1489
产品类型：流式抗体
品牌：eBioscience
抗原：CD19
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：eBio1D3 (1D3)
荧光染料：Super Bright 702
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 应用领域： | Flow |
| 推荐用法： | 0.5 µg/test（flow) |
| The eBio1D3 (1D3) monoclonal antibody reacts with mouse CD19, a 95 kDa transmembrane glycoprotein. CD19 is expressed by B cells during all stages of development excluding the terminally differentiated plasma cells. Follicular dendritic cells also express CD19. Together CD21, CD81, MHC class II, and CD19 form a multimolecular complex that associates with the BCR. Signaling through CD19 induces tyrosine phosphorylation, calcium flux and proliferation of B cells.Applications Reported: This eBio1D3 (1D3) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBio1D3 (1D3) antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 702 nm; Laser: Violet Laser |
| 数据 |
CD19 Antibody (67-0193-82) in Flow BALB/c mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC (Product # 11-0031-82) and of Rat IgG2a kappa Isotype Control, Super Bright 702 (Product # 67-4321-82) (left) or of CD19 Monoclonal Antibody, Super Bright 702 (right). Cells in the lymphocyte gate were used for analysis. |
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| 参考文献： |
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