CD4 Monoclonal Antibody (RM4-5), Super Bright 780, eBioscience™
货号：78-0042-82
规格：100 μg
价格：3293
产品类型：流式抗体
品牌：eBioscience
抗原：BALB/c mouse thymocytes
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：RM4-5
荧光染料：Super Bright 780
| 抗体类型: | 单抗 | 同型对照： | Rat IgG2a kappa Isotype Control (eBR2a), Super Bright 780 |
| 免疫原性： | | 应用领域： | Flow |
| 推荐用法： | 0.125 µg/test（flow) |
| Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: The GK1.5 antibody has been reported for use in flow cytometric analysis. It has also been reported in isolation and depletion of CD4+ cells, blocking of ligand binding and T cell activation via CD4.Applications Tested: The GK1.5 antibody has been tested by flow cytometric analysis of mouse thymocytes and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (Product # 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (Product # 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 405 nm; Emission: 780 nm; Laser: Violet Laser |
| 数据 |
CD4 Antibody (78-0042-82) in Flow C57BL/6 mouse splenocytes were stained with CD3e Monoclonal Antibody, FITC (Product # 11-0031-82) and 0.25 µg of Rat IgG2a kappa Isotype Control, Super Bright 780 (Product # 78-4321-82) (left) or 0.25 µg of CD4 Monoclonal Antibody, Super Bright 780 (right). Total viable cells were used for analysis, as determined by 7-AAD (Product # 00-6993-50). |
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| 参考文献： |
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