IntracellularFixationPermeabilizationBufferSet流式细胞胞内固定破膜液

货号：88-8824-00

规格：1 kit

价格：1802

产品类型：固定破膜剂

品牌：eBioscience

物种：其它

宿主：其它

抗体亚型：其它

荧光染料：其它

tablecellspacing0cellpadding0tbodytrclassfirstRowtdvaligntopstyleborder-width1pxpadding5px10pxword-breakbreak-allwidth993pstylemargin-bottom0pxline-height24pxspanstylefont-size14pxfont-familyarialhelveticasans-serifspanstylefont-size14pxcolorrgb515151background-colorrgb255255255IntracellularFixationampPermeabilizationBufferSet被设计用于细胞内染色与流式细胞分析并经专门配方以减少被荧光染料标记抗体的非特异性结合提高荧光信噪比spanspanppstylemargin-bottom0pxline-height24pxspanstylefont-size14pxfont-familyarialhelveticasans-serifspanstylefont-size14pxcolorrgb515151background-colorrgb255255255第一步活细胞经固定缓冲液固定使得蛋白交联spanspanppstylemargin-bottom0pxline-height24pxspanstylefont-size14pxfont-familyarialhelveticasans-serifspanstylefont-size14pxcolorrgb515151background-colorrgb255255255第二步用破膜缓冲液破膜以使抗体染料能有效进入细胞spanspanppstylemargin-bottom0pxline-height24pxspanstylefont-size14pxfont-familyarialhelveticasans-serifspanstylefont-size14pxcolorrgb515151background-colorrgb255255255随后的抗体染色步骤加入抗体并采用破膜缓冲液在破膜后进行孵育spanspanppstylemargin-bottom0pxline-height24pxspanstylefont-size14pxfont-familyarialhelveticasans-serifspanstylefont-size14pxcolorrgb515151background-colorrgb255255255最终清洗步骤以及样品检测前使用FlowCytometryStainingBuffer货号ahrefhttpwwwnwbioteccomindexphpgmProductaprdtid3035target_self00-4222a制备细胞悬液spanspanppstylemargin-bottom0pxline-height24pxspanstylefont-size14pxfont-familyarialhelveticasans-serifspanstylefont-size14pxcolorrgb515151background-colorrgb255255255brspanspanppstylemargin-bottom0pxline-height24pxspanstylefont-size14pxfont-familyarialhelveticasans-serifspanstylefont-size14pxcolorrgb515151background-colorrgb255255255该试剂盒被广泛用于细胞质蛋白以及分泌蛋白如趋化因子和细胞因子的染色本品不建议用于细胞核内蛋白的染色如进行核内染色请考虑Foxp3TranscriptionFactorBufferSet货号ahrefhttpwwwnwbioteccomindexphpgmProductaprdtid3037target_self00-5523aspanbrstylecolorrgb515151font-familyquotHelveticaNeuequotHelveticaRobotoquotSegoeUIquotArialsans-seriffont-size13pxwhite-spacenormalbackground-colorrgb255255255brstylecolorrgb515151font-familyquotHelveticaNeuequotHelveticaRobotoquotSegoeUIquotArialsans-seriffont-size13pxwhite-spacenormalbackground-colorrgb255255255spanstylefont-size14pxcolorrgb515151background-colorrgb255255255进行固定步骤之前建议使用一种FixableViabilityDyeseFluor450506660780520或455UV标记活细胞然后继续进行固定spanspanspanstylefont-size14pxspanppstylemargin-bottom0pxline-height24pxbrptdtrtrtdvaligntopstyleborder-right-width1pxborder-bottom-width1pxborder-left-width1pxborder-topnonebackgroundrgb242242242padding5px10pxwidth992pstylemargin-bottom0strongspanstylefont-family宋体相关产品spanstrongptdtrtrtdvaligntopstyleborder-right-width1pxborder-bottom-width1pxborder-left-width1pxborder-topnonepadding5px10pxwidth992h4stylemargin-top5pxmargin-right0margin-bottom10pxmargin-left0strongspanstylefont-size14pxfont-familyArialsans-seriffont-weightnormalnbspspanstrongahrefhttpwwwnwbioteccomindexphpgmProductaprdtid3037target_selfstrongspanstylefont-size14pxfont-familyArialsans-seriffont-weightnormalnbspspanstrongstrongstylecolorinheritfont-familyHelveticaNeue-LightquotHelveticaNeueLightquotquotHelveticaNeuequotHelveticaRobotoquotSegoeUIquotArialsans-seriffont-size2808pxletter-spacing1pxspanstylefont-size14pxfont-familyarialhelveticasans-serifeBioscienceFoxp3TranscriptionFactorStainingBufferSet货号00-5523-00spanstrongah4h4stylemargin-top5pxmargin-right0margin-bottom10pxmargin-left0strongspanstylefont-size14pxfont-familyArialsans-seriffont-weightnormalnbspnbspspanstrongahrefhttpnwbioteccomindexphpgmProductaprdtid3035target_selfstrongstylecolorinheritfont-familyHelveticaNeue-LightquotHelveticaNeueLightquotquotHelveticaNeuequotHelveticaRobotoquotSegoeUIquotArialsans-seriffont-size2808pxletter-spacing1pxspanstylefont-size14pxfont-familyarialhelveticasans-serifeBioscienceFlowCytometryStainingBuffer货号00-4222-2657spanstrongah4h4stylemargin-top5pxmargin-right0margin-bottom10pxmargin-left0strongspanstylefont-size14pxfont-familyArialsans-seriffont-weightnormalnbspspanstrongh4tdtrtbodytablepbrp

技术参数

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