Cell Stimulation Cocktail 细胞激活剂（PMA和离子霉素）(500X)/广谱激活剂
货号：00-4970-03,00-4970-93
规格：4 x 100 µL,100 µL
价格：3064,1065
产品类型：免疫细胞培养
品牌：eBioscience
| 描述eBioscience™ Cell Stimulation Cocktail (500X) 是佛波醇酯 12-十四酸酯 13-乙酸酯 (PMA) 和离子霉素的混合物。PMA激活Protein Kinase C, 离子霉素Ionomycin 促进Ca2+穿膜运动，使用 PMA 和离子霉素进行处理足以诱导大多数细胞类型激活，使得CD69, CD154表达升高，产生细胞因子：IL-2、IFN-γ和TNF-α。这种混合物用于诱导体外细胞培养物产生细胞因子，可使用免疫检测法进行后续检测。它还可与 Protein Transport Inhibitor Cocktail(500X)（货号#录00-4980）联用以测定诱导细胞因子表达的细胞内保留量。 |
| 数据： |
图1 Data for Cell Stimulation Cocktail (500X)Mouse splenocytes were cultured with 1X Cell Stimulation Cocktail and 1X Protein Transport Inhibitor Cocktail (cat. 00-4980) for 16 hours. Cultures were harvested and fixed and permeabilized with IC Fixation Buffer (cat. 00-8222) and Permeabilization Buffer (cat. 00-8333). The cells were then stained with Anti-Mouse CD4 FITC (cat. 11-0042), Anti-Mouse IFN gamma PE (12-7311) and Anti-Mouse TNF alpha APC (cat. 17-7321). Cells in the lymphocyte gate were used for analysis. |
| 相关产品： |
| ▪eBioscience™ Phytohemagglutinin-L (PHA-L) Solution (500X)（货号#00-4977-03/00-4977-93）▪eBioscience™ Lipopolysaccharide (LPS) Solution (500X)（货号#00-4976-03/00-4976-93）▪eBioscience™ Concanavalin A (Con A) Solution (500X)（货号#00-4978-03/00-4978-93）▪eBioscience™ Cell Stimulation Cocktail (plus protein transport inhibitors) (500X)（货号#00-4975-03/00-4975-93） |
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