CD3 Monoclonal Antibody (HIT3a), 功能抗体Functional Grade, eBioscience™

货号：16-0039-81,16-0039-85

规格：50 μg,500 μg

价格：1328,3911

产品类型：流式抗体

品牌：eBioscience

抗原：CD3

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：HIT3a

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG2a, kappa
免疫原性：		用法：	0.25 µg/test(Flow）

The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis.Applications Tested: The HIT3a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationThe CD3 subunit complex which is crucial in transducing antigen-recognition signals into the cytoplasm of T cells and in regulating the cell surface expression of the TCR complex. T cell activation through the antigen receptor (TCR) involves the cytoplasmic tails of the CD3 subunits CD3 gamma, CD3 delta, CD3 epsilon and CD3 zeta. These CD3 subunits are structurally related members of the immunoglobulins super family encoded by closely linked genes on human chromosome 11. The CD3 components have long cytoplasmic tails that associate with cytoplasmic signal transduction molecules and this association is mediated at least in part by a double tyrosine-based motif present in a single copy in the CD3 subunits. CD3 may play a role in TCR-induced growth arrest, cell survival and proliferation. The CD3 antigen is present on 68-82% of normal peripheral blood lymphocytes, 65-85% of thymocytes and Purkinje cells in the cerebellum. It is never expressed on B or NK cells. Decreased percentages of T lymphocytes may be observed in some autoimmune diseases. The genes encoding the CD3 epsilon, gamma and delta polypeptides are located on chromosome 11. Defects in the CD3 gene are associated with CD3 immunodeficiency.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD3 Antibody (16-0039-81) in FlowStaining of normal human peripheral blood cells with Anti-Human CD3 PE.Autofluorescence is indicated by open histogram.Cells in the lymphocyte populationwere used for analysis.

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参考文献：

1.PloS oneLarge-Scale Purification of r28M: A Bispecific scFv Antibody Targeting Human Melanoma Produced in Transgenic Cattle."16-0039 was used in an ELISA assay to study how the bispecific scFv antibody r28M produced in transgenic cattle targets human melanoma."Authors Spiesberger K,Paulfranz F,Egger A,Reiser J,Vogl C,Rudolf-Scholik J,Mayrhofer C,Grosse-Hovest L,Brem G2.The Journal of biological chemistryHIV-1 clade B Tat, but not clade C Tat, increases X4 HIV-1 entry into resting but not activated CD4+ T cells."16-0039 was used in Functional assays to investigate differences in effects of Tat secreted by different HIV clades."Authors Campbell GR,Loret EP,Spector SA3.FEBS lettersRegulation of growth and survival of activated T cells by cell-transducing inhibitors of Ras."16-0039 was used in Functional assays to develop cell-penetrating Ras inhibitors and to study their ability to inhibit T-cell activation, showing that anti-CD3/CD28-activated cells had a reduction in cell size, proliferation and increased apoptosis; revealing a role of Ras in survival in T cells."

技术参数

产品应用 Flow；FN；ELISA

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