YO-PRO™-1 Iodide (491/509)
货号：Y3603
规格：1 mL
价格：5736
产品类型：细胞凋亡检测
品牌：Thermo Fisher
物种：恒河猴/小鼠/大鼠
宿主：小鼠
抗体亚型：IgG
荧光染料：eFluor 506
| 发绿色荧光的 YOPRO® -1染色剂是我们独有的碳氰酸酯核酸染色剂之一，经证是标记凋亡细胞的利器。凋亡细胞对 YO-PRO®-1具有通透性，但对死细胞染色剂碘化丙啶 (P-3566) 仍无通透性。活细胞不会被 YO-PRO®-1染色，可在后续实验中继续使用。我们的 Vybrant® 细胞凋亡检测试剂盒 #4 (V-13243) 和 #7 (V-23201) 之中也含有核酸染色剂。 |
| 数据： |
图1.Jurkat cells. YO-PRO®-1 dye and propidium iodide, components of the Membrane Permeability/Dead Cell Apoptosis Kit.Apoptosis induced in Jurkat cells with 10 µM camptothecin. The cells were then treated with the reagents in the Membrane Permeability/Dead Cell Apoptosis Kit (Cat. no. V13243). Apoptotic cell nuclei were labeled with YO-PRO®-1 dye (green) (Cat. no. Y3603). Necrotic cells were detected with propidium iodide (red) (Cat. no. P1304MP, P3566, P21493).图2.Macrophages cultured on a polymer surface that have fused to form a foreign-body giant cell following treatment with interleukin-4.Macrophages cultured on a polymer surface that have fused to form a foreign-body giant cell following treatment with interleukin-4. Cells were fixed with 3.7% formaldehyde, treated with RNase A and stained with rhodamine phalloidin (Cat. no. R415) to visualize F-actin, and with YO-PRO®-1 iodide (Cat. no. Y3603) to visualize cell nuclei. Cells were imaged with a Bio-Rad MRC600 confocal laser-scanning microscope. The image is a composite of optical sections taken through the Z-axis of the cell. F-actin (red) is restricted to the periphery of the multinucleated cell and surrounds a cluster of nuclei (green). Image contributed by Kristin DeFife and James M. Anderson, Institute of Pathology, Case Western Reserve University. |
| 相关产品： |
| ▪细胞周期检测▪细胞凋亡检测▪细胞增殖活性检测 |
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