| Description: The HIT3a monoclonal antibody reacts with human CD3e, a 20 kDa subunit of the TCR complex. Along with the other CD3 subunits gamma and delta, the epsilon chain is required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR with HIT3a initiates an intracellular biochemical pathway resulting in cellular activation and proliferation.Applications Reported: The HIT3a antibody has been reported for use in flow cytometric analysis.Applications Tested: The HIT3a antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.The Journal of experimental medicineAnti-TNF drives regulatory T cell expansion by paradoxically promoting membrane TNF-TNF-RII binding in rheumatoid arthritis.2016"14-0039 was used in Functional assays to investigate whether TNF boosts of inhibits regulatory T cells (T reg cells), showing that anti-TNF drives regulatory T cell expansion by promoting membrane TNF-TNF-RII binding, in rheumatoid arthritis."Authors Nguyen DX,Ehrenstein MR2.Journal of immunology (Baltimore, Md. : 1950)Cutting edge: circulating plasmablasts induce the differentiation of human T follicular helper cells via IL-6 production.2015"14-0039 was used in Flow cytometry/Cell sorting to identify a reciprocal interaction between circulating human plasmablasts and Tfh cells which drives Tfh cell differentiation."Authors Chavele KM,Merry E,Ehrenstein MR3.PloS onePotential role of IL-17-producing iNKT cells in type 1 diabetes.2015"14-0039 was used in Flow cytometry/Cell sorting to investigate the status and potential role of iNKT17 in type 1 diabetes."Authors Li S,Joseph C,Becourt C,Klibi J,Luce S,Dubois-Laforgue D,Larger E,Boitard C,Benlagha K4.Epigenetics and chromatinGeneration of bivalent chromatin domains during cell fate decisions.2011"14-0039 was used in Flow cytometry/Cell sorting to investigate the patterns and levels of histone modifications at bivalent genes, showing the generation of bivalent chromatin domains during cell fate decisions."Authors De Gobbi M,Garrick D,Lynch M,Vernimmen D,Hughes JR,Goardon N,Luc S,Lower KM,Sloane-Stanley JA,Pina C,Soneji S,Renella R,Enver T,Taylor S,Jacobsen SE,Vyas P,Gibbons RJ,Higgs DR |
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