M-CSF (Macrophage colony-stimulating factor)
货号：PHP220
规格：10 µg
价格：3162
产品类型：细胞因子添加物
品牌：Bio-Rad
| 产品介绍 | | |
| M-CSF (Macrophage colony-stimulating factor)is a potent lineage restricted haematopoietic growth factor and member of the CSF family of hormone-like glycoproteins, which plays a key role in the regulation of cellular differentiation, proliferation, and the survival of blood monocytes, tissue macrophages and their progenitor cells.Along with sRANKL/CD254/OPGL, M-CSF is essential for osteoclastogenesis, acting through the tyrosine kinase receptor c-fms (colony stimulating factor 1 receptor), and facilitates haematopoietic recovery following bone marrow transplantation. |
| 产品详情 | | |
| Target Species | Human |
| Product Form | Purified recombinant protein - lyophilized |
| Preparation | Purified recombinant M-CSF expressed inE.coli |
| Reconstitution | Reconstitute with 0.1 ml distilled water. For extended storage the addition of 0.1% BSA is recommended.Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. Bio-Rad recommend that the vial is gently mixed after reconstitution. |
| Activity | The ED50, determined by the dose-dependent stimulation of the proliferation of M-NSF-60 cells, was ≤ 1 ng/ml corresponding to a specific activity of ≥ 1 x 106units/mg. |
| Preservative Stabilisers | None present |
| Approx. Protein Concentrations | 0.1 mg/ml after reconstitution |
| Protein Molecular Weight | 36.8 kDa (159 amino acid homodimer) |
| Purity | ≥ 98% by SDS PAGE and HPLC |
| Regulatory | For research purposes only |
| Guarantee | 3 months from date of reconstitution |
| 存储条件 | | |
| Prior to reconstitution store at +4℃.After reconstitution store at -20℃.Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use. |
| 应用 | | |
| Application Name | Verified | Min Dilution | Max Dilution |
| Functional Assays | √ | 1.0 | |
| Where this product has not been tested for use in a particular technique this does not necessarily exclude its use in such procedures. Suggested working dilutions are given as a guide only. It is recommended that the user titrates the product for use in their own system using appropriate negative/positive controls. |
技术参数 产品应用 wb，elisa
产品类型 细胞因子添加物
推荐用于 wb，elisa
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