Description: The OKT3 monoclonal antibody reacts with an epitope on the epsilon-subunit within the human CD3 complex. The OKT3 antibody has been reported to have potent immunosuppressive properties in vivo and has been proven effective in the treatment of renal, heart and liver allograft rejection. The CD3 subunits, gamma, delta, and epsilon chains, are required for proper assembly, trafficking and surface expression of the TCR complex. CD3 is expressed by thymocytes in a developmentally regulated manner and by all mature T cells. Crosslinking of TCR initiates an intracellular biochemical pathway resulting in cellular activation and proliferation. Applications Reported: This OKT3 antibody has been reported for use in flow cytometric analysis. Applications Tested: This OKT3 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome. Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser. Filtration: 0.2 µm post-manufacturing filtered. |
| 1.JCI insightB cells from African American lupus patients exhibit an activated phenotype."48-0037 was used in Flow cytometry/Cell sorting to investigate immunological differences between African American and European American patients with systemic lupus erythematosus, showing that B cells from African American patients exhibit an activated phenotype."Authors Menard LC,Habte S,Gonsiorek W,Lee D,Banas D,Holloway DA,Manjarrez-Orduno N,Cunningham M,Stetsko D,Casano F,Kansal S,Davis PM,Carman J,Zhang CK,Abidi F,Furie R,Nadler SG,Suchard SJ2.Frontiers in immunologyTertiary Lymphoid Organs in Takayasu Arteritis."48-0037 was used in Flow cytometry/Cell sorting to investigate the role of B cells and tertiary lymphoid organs in the pathogenesis of Takayasu arteritis."Authors Clement M,Galy A,Bruneval P,Morvan M,Hyafil F,Benali K,Pasi N,Deschamps L,Pellenc Q,Papo T,Nicoletti A,Sacre K3.PloS oneNeuropilin-1 expression characterizes T follicular helper (Tfh) cells activated during B cell differentiation in human secondary lymphoid organs."48-0037 was used in Flow cytometry/Cell sorting to characterise Nrp1-expressing T cells in human secondary lymphoid organs."Authors Renand A,Milpied P,Rossignol J,Bruneau J,Lemonnier F,Dussiot M,Coulon S,Hermine O4.Blood cancer journalA novel antibody-drug conjugate targeting SAIL for the treatment of hematologic malignancies."48-0037 was used in Flow cytometry/Cell sorting to describe the proteomic identification of a novel cancer target, SAIL."Authors Kim SY,Theunissen JW,Balibalos J,Liao-Chan S,Babcock MC,Wong T,Cairns B,Gonzalez D,van der Horst EH,Perez M,Levashova Z,Chinn L,D'Alessio JA,Flory M,Bermudez A,Jackson DY,Ha E,Monteon J,Bruhns MF,Chen G,Migone TS5.LeukemiaEpigenomic analysis of the HOX gene loci reveals mechanisms that may control canonical expression patterns in AML and normal hematopoietic cells."48-0037 was used in Flow cytometry/Cell sorting to analyse RNA sequencing data from primary AML samples and normal haematopoietic cells in order to compare HOX expression in normal and leukaemic cells."Authors Spencer DH,Young MA,Lamprecht TL,Helton NM,Fulton R,O'Laughlin M,Fronick C,Magrini V,Demeter RT,Miller CA,Klco JM,Wilson RK,Ley TJ |
QQ:1749072012