ANTI-HUMAN CD45 (HI30) PE-CY5.5
货号：35-0459-41,35-0459-42
规格：25T,100T
价格：1258,3141
产品类型：流式抗体
品牌：eBioscience
抗原：CD45
物种：人
宿主：小鼠
抗体亚型：IgG
克隆号：HI30
荧光染料：PE-Cy5.5
| 抗体类型: | 单抗 | 同型对照： | Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), PE-Cyanine5.5 |
| 免疫原性： | | 用法： | 5uL（0.03 µg）/test(Flow） |
| Description: The HI30 monoclonal antibody reacts with all isoforms of human CD45, also known as Leukocyte Common Antigen (LCA). CD45 is expressed by all hematopoietic cells excluding circulating erythrocytes and platelets. The cytoplasmic portion of CD45 has tyrosine phosphatase enzymatic activity and plays an important role in activation of lymphocytes.Applications Reported: This HI30 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI30 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.03 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Light sensitivity: This tandem dye is sensitive photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-567 nm; Emission: 690 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser |
| 数据 |
CD45 Antibody (35-0459-41) in FlowStaining of normal human peripheral blood cells with Mouse IgG1 K Isotype Control PE-Cyanine5-5 (Product # 35-4714-82) (blue histogram) or Anti-Human CD45 PE-Cyanine5-5 (purple histogram). Cells in the lymphocyte gate were used for analysis. |
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