| Description: The 4H11 monoclonal antibody reacts with human CD34, also known as mucosialin. CD34 belongs to a protein family which also includes endoglycan and podocalyxin. Members of this family are single pass transmembrane proteins with a heavily glycosylated extracellular and N-terminal mucin domain. CD34 was first identified as an antigen expressed on hematopoietic progenitors, and has since been extensively used as a marker to isolate cells capable of hematopoietic cell engraftment. In spite of this, the function of CD34 remains unresolved. In addition to expression on hematopoietic progenitors, CD34 is expressed on some populations of mesenchymal stem cells, tumor cell lines, and by vascular endothelia in the adult. Epitopes of CD34 have been assigned to three classes (class I, II or III) based on their differential sensitivity to enzymatic cleavage by neuraminidase, chymopapain, or O-glycoprotease. According to this analysis, the 4H11 antibody belongs to class III, indicating that it reacts with a protein epitope.Applications Reported: This 4H11 (APG) antibody has been reported for use in flow cytometric analysis.Applications Tested: This 4H11 (APG) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (1 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.eFluor® 450 is an alternative to Pacific Blue®. eFluor® 450 emits at 445 nm and is excited with the Violet laser (405 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 405 nm; Emission: 445 nm; Laser: Violet Laser.Filtration: 0.2 µm post-manufacturing filtered. |
| 1.Nature communicationsCD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity."48-0349 was used in Flow cytometry/Cell sorting to study how CD19 CAR immune pressure induces a B-precursor acute lymphoblastic leukaemia lineage switch."Authors Jacoby E,Nguyen SM,Fountaine TJ,Welp K,Gryder B,Qin H,Yang Y,Chien CD,Seif AE,Lei H,Song YK,Khan J,Lee DW,Mackall CL,Gardner RA,Jensen MC,Shern JF,Fry TJ2.American journal of physiology. Cell physiologyFunctional plasticity of the N-methyl-d-aspartate receptor in differentiating human erythroid precursor cells."48-0349 was used in Flow cytometry/Cell sorting to characterse the changes in Ca(2+) uptake and electric currents mediated by the NMDARs occurring during erythroid precursor cell differentiation."Authors Hänggi P,Telezhkin V,Kemp PJ,Schmugge M,Gassmann M,Goede JS,Speer O,Bogdanova A3.NeoplasmaGrowth inhibitory effect of the antibody to hematopoietic stem cell antigen CD34 in leukemic cell lines.Authors Elknerová K,Lacinová Z,Soucek J,Marinov I,Stöckbauer P |
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