Goat anti-Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor Plus 555/羊抗鼠 IgG (H+L) 高交叉吸附荧光二抗，Alexa Fluor Plus 555

货号：A32727

规格：1 mg

价格：3832

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor plus 555

36个不同种属、不同荧光的Alexa Fluor plus荧光二抗点击表格中的货号，可跳转到相应的产品页。

	AF Plus Secondary Antibody products
	AF Plus 488	AF Plus 555	AF Plus 594	AF Plus 647	AF Plus 680	AF Plus 800
羊抗兔	A32731	A32732	A32740	A32733	A32734	A32735
羊抗鼠	A32723	A32727	A32742	A32728	A32729	A32730
羊抗鸡	A32931	A32932	A32759	A32933	A32934	A32935
驴抗羊	A32814	A32816	A32758	A32849	A32860	A32930
驴抗兔	A32790	A32794	A32754	A32795	A32802	A32808
驴抗鼠	A32766	A32773	A32744	A32787	A32788	A32789

产品优势：1. 更多荧光选择（从480到800），更多种属选择；2. 更好的光稳定性，更高信噪比，更高灵敏度，经过交叉吸附检测；3. 发布1年多已经有多达99篇高分文章引用；4. 适用于ICC/IF/WB等实验应用。

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1-10µg/mL(ICC);1-10µg/mL（IF);0.1-0.4µg/mL(WB)

产品详细信息To minimize cross-reactivity, the goat anti-mouse IgG whole antibodies have been pre cross-adsorbed against bovine IgG, goat IgG, rabbit IgG, rat IgG, human IgG, and human serum. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in less background staining and cross-reactivity. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. Further passages through additional columns result in highly cross-adsorbed preparations of secondary antibody. The benefits of these extra steps are apparent in multiplexing/multicolor-staining experiments where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically.靶标信息Obtain superior images with the new Invitrogen™ Alexa Fluor™ Plus secondary antibodies. Make your low abundant targets visible, minimize time spent optimizing, and make your precious samples count. Alexa Fluor™ Plus secondary antibodies provide brighter signal while having lower cross-reactivity compared to leading Alexa Fluor secondary antibodies, providing you with higher sensitivity and better signal-to-noise for your critical experiments.Alexa Fluor Plus secondary antibodies represent an advancement in fluorescent conjugate technology. Alexa Fluor Plus secondary antibodies are conjugated using new, proprietary dye chemistry and are pre-adsorbed to reduce background and cross-reactivity so you can generate stunning images. Alexa Fluor Plus secondary antibodies provide up to 4.2 fold higher signal-to-background in imaging applications and up to 5.8 fold higher signal-to-background in fluorescent western applications compared to Alexa Fluor secondary conjugates.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32727) in IFImmunofluorescent analysis of glial fibrillary acidic protein (GFAP) in E18 Sparague Dawley primary cortical neuronal cells containing astrocytes. The cells were fixed with 4% formaldehyde for 15 mins, permeabilized with 0.25% Triton X-100 in PBS for 10 mins, and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a GFAP mouse monoclonal antibody (Product # MA5-12023) at a dilution of 1:200 in 3% BSA in PBS for 1 hr at RT, and then incubated with Invitrogen Alexa Fluor Plus 555 goat anti-mouse IgG secondary antibody (Product # A32727) at a dilution of 1:1000 for 1 hr at RT. Nuclei were stained with Hoechst 33342 (product # H3570). The image contains overlay of GFAP (orange) and nuclei (blue). Images were taken on a Zeiss LSM 710 confocal microscope at 40X magnification. Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32727) in IFImmunofluorescent analysis of Cytochrome C in A549 cells. The cells were fixed with 4% formaldehyde for 15 mins, permeabilized with 0.25% Triton X-100 in PBS for 10 mins, and blocked with 3% BSA in PBS for 30 mins at RT. Cells were stained with a Cytochrome C mouse monoclonal antibody (Product # 33-8200) at a dilution of 1:200 in 3% BSA in PBS for 1 hr at RT, and then incubated with Invitrogen Alexa Fluor Plus 555 goat anti-mouse IgG secondary antibody (Product # A32727) at a dilution of 1:1000 for 1 hr at RT. Nuclei were stained with Hoechst 33342 (Product # H3570). The image contains overlay of Cytochrome C (orange) and nuclei (blue). Images were taken on a Zeiss LSM 710 confocal microscope at 40X magnification. Mouse IgG (H+L) Highly Cross-Adsorbed Secondary Antibody (A32727) in WBWestern blot analysis of Heat Shock Protein 60 (Hsp60) and Actin was performed by loading 3-fold serial dilutions of A431 whole cell lysate (starting at 15ug) and 2ul of the PageRuler Prestained NIR Protein Ladder (Product # 26635) per well onto a 4-20% Tris-HCl polyacrylamide gel. Proteins were transferred to Nitrocellulose Membranes (Product # 88018) and blocked with Fluorescence Blocker for 30 min. Membranes were probed with a Hsp60 monoclonal antibody (Product # MA3-012) at a dilution of 1:500 and a Actin monoclonal antibody at a dilution of 1:1000 (Product # MA1-744) overnight at 4°C on a rocking platform, washed with TBST, and probed with an Invitrogen Alexa Fluor Plus 555 Goat anti-Mouse IgG secondary antibody (Product # A32727) at dilutions of 1:10,000 for 45 minutes. Blots were imaged on a fluorescence imaging system.

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技术参数

产品优点 1.特异性强；2.亮度高，耐淬灭，对PH值不敏感；3.能高灵敏的检测低丰度蛋白。

产品应用 ICC；IF；WB

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