CD44 Monoclonal Antibody (IM7), PE, eBioscience™

货号：12-0441-81,12-0441-82,12-0441-83

规格：50 µg,100 µg,200 µg

价格：848,1358,2084

产品类型：流式抗体

品牌：eBioscience

抗原：CD44

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：IM7

荧光染料：PE

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PE
免疫原性：		用法：	0.125µg/test(Flow）

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: The IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: The IM7 antibody has been tested by flow cytometric analysis of mouse bone marrow cells and splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD44 Antibody (12-0441-81) in FlowDifferential expression of Anti-Human/Mouse CD44 PE on mouse bone marrow cells.BALB/c bone marrow cells were stained with 0.06 µg of Anti-Human/Mouse CD44 PE.Total viable cells were gated further on their light scatter profile. The lymphoid lineagecells (R2) express Anti-Human/Mouse CD44 PE at an intermediate level, while the myeloidlineage cells (R3 and R4) display much higher levels of Anti-Human/Mouse CD44 PE surfaceexpression. The overlay histogram on each panel (green) is the autofluorescence of cells.

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参考文献：

1.The Journal of clinical investigationLoss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms."12-0441 was used in Flow cytometry/Cell sorting to identify pleckstrin-2 as an effector of the JAK2/STAT5 pathway and a key factor in the pathogenesis of JAK2V617F-induced myeloproliferative neoplasms."Authors Zhao B,Mei Y,Cao L,Zhang J,Sumagin R,Yang J,Gao J,Schipma MJ,Wang Y,Thorsheim C,Zhao L,Stalker T,Stein B,Wen QJ,Crispino JD,Abrams CS,Ji P2.Cellular and molecular life sciences : CMLSOpposing effects on the cell cycle of T lymphocytes by Fbxo7 via Cdk6 and p27."12-0441 was used in Flow cytometry/Cell sorting to test the physiological role of an F-box protein, Fbxo7, in multiple tissues."Authors Patel SP,Randle SJ,Gibbs S,Cooke A,Laman H3.JCI insightCeramide synthesis regulates T cell activity and GVHD development."12-0441 was used in Flow cytometry/Cell sorting to define the involvement of ceramides in the T-cell response to alloantigens present after allogeneic haematopoietic cell transplantation."Authors Sofi MH,Heinrichs J,Dany M,Nguyen H,Dai M,Bastian D,Schutt S,Wu Y,Daenthanasanmak A,Gencer S,Zivkovic A,Szulc Z,Stark H,Liu C,Chang YJ,Ogretmen B,Yu XZ4.Nature communicationsPhotothermal therapy with immune-adjuvant nanoparticles together with checkpoint blockade for effective cancer immunotherapy."12-0441 was used in Immunohistochemistry to investigate the use of immuneadjuvant nanoparticles for photothermal tumour ablation in mice and their potential for cancer immunotherapy."Authors Chen Q,Xu L,Liang C,Wang C,Peng R,Liu Z5.Nature communicationsTreg engage lymphotoxin beta receptor for afferent lymphatic transendothelial migration."12-0441 was used in Magnetic cell separation to studying Treg cell engagement with the lymphotoxin beta receptor in transendothelial migration."Authors Brinkman CC,Iwami D,Hritzo MK,Xiong Y,Ahmad S,Simon T,Hippen KL,Blazar BR,Bromberg JS

技术参数

产品应用 Flow；ICC；IF；MISC

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