SSEA1 Monoclonal Antibody (eBioMC-480 (MC-480)), PerCP-eFluor 710, eBioscience™
货号：46-8813-42
规格：100 Tests
价格：3760
产品类型：流式抗体
品牌：eBioscience
抗原：SSEA1
物种：人/小鼠
宿主：小鼠
抗体亚型：IgM
克隆号：eBioMC-480 (MC-480) Conjugate PerCP-eFluor™ 710
荧光染料：PerCP-eFluor™ 710
| 抗体类型: | 单抗 | 同型对照： | IgM |
| 免疫原性： | | 用法： | 0.125 µg/test(Flow） |
Description: The eBioMC-480 (MC-480) antibody reacts with the stage-specific embryonic antigen-1 (SSEA-1), a carbohydrate epitope expressed upon the surface of early mouse embryos, murine embryonal carcinoma cells (EC), murine embryonic stem cells (ES) and murine and human germ cells (EG). No immunoreactivity is evident with undifferentiated human EC and ES cells. Differentiation of human EC results in an increase in SSEA-1 expression, while in the mouse expression is diminished. Expression of the carbohydrate moiety is also found on mature human granulocytes (on CD15) and some monocytes. SSEA-1 is associated with cell adhesion, migration and differentiation.Applications Reported: This eBioMC-480 (MC-480) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioMC-480 (MC-480) antibody has been pre-titrated and tested by flow cytometric analysis of F9 cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.PerCP-eFluor® 710 can be used in place of PE-Cy5, PE-Cy5.5 or PerCP-Cy5.5. PerCP-eFluor® 710 emits at 710 nm and is excited with the blue laser (488 nm). Please make sure that your instrument is capable of detecting this fluorochrome. For a filter configuration, we recommend using the 685 LP dichroic mirror and 710/40 band pass filter, however the 695/40 band pass filter is an acceptable alternative.Our testing indicates that PerCP-eFluor® 710 conjugated antibodies are stable when stained samples are exposed to freshly prepared 2% formaldehyde overnight at 4°C, but please evaluate for alternative fixation protocols.Excitation: 488 nm; Emission: 710 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
| SSEA1 Antibody (46-8813-41) in Flow Staining of F9 cells with staining buffer (autofluorescence) (blue histogram) or Anti-Human/Mouse SSEA-1 PerCP-eFluor® 710 (purple histogram). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.NatureParent stem cells can serve as niches for their daughter cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer2.PLoS geneticsCell reprogramming requires silencing of a core subset of polycomb targets.View published figure on By clicking the link above, you will be redirected to the Benchsci website. Disclaimer3.Nature cell biologyEmbryonic stem cells require Wnt proteins to prevent differentiation to epiblast stem cells.View published figure on By clicking the link above, you will be redirected to the Benchsci website. DisclaimerA |
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