CD140b (PDGFRB) Monoclonal Antibody (APB5), PE, eBioscience™
货号：12-1402-81
规格：50 µg
价格：1733
产品类型：流式抗体
品牌：eBioscience
抗原：CD140b (PDGFRB)
物种：小鼠
宿主：大鼠
抗体亚型：IgG
克隆号：APB5
荧光染料：PE
| 抗体类型: | 单抗 | 同型对照： | IgG2a, kappa |
| 免疫原性： | | 用法： | 1 µg/test(Flow） |
Description: The APB5 monoclonal antibody reacts with the mouse CD140b molecule, the beta chain of the platelet derived growth factor receptor (PDGF receptor). PDGFRb is a receptor tyrosine kinase that forms dimers on the surface upon ligand binding and phosphorylates substrates. Dimers of PDGFR consist of either homodimers of alpha/alpha, beta/beta, or heterodimers of alpha/beta and serve as a substrate for its kinase activity. CD140b is expressed by embryonic tissues and mesenchymal-derived cells of the adult mouse tissues. The PDGFR beta chain is reported to play a significant role in formation of fibrous atherosclerotic lesions.Applications Reported: This APB5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This APB5 antibody has been tested by flow cytometric analysis of NIH/3T3 cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationPDGFRb is a cell surface tyrosine kinase receptor for members of the platelet-derived growth factor family. These growth factors are mitogens for cells of mesenchymal origin. The identity of the growth factor bound to a receptor monomer determines whether the functional receptor is a homodimer or a heterodimer, composed of both platelet-derived growth factor receptor alpha and beta polypeptides. The gene is flanked on chromosome 5 by the genes for granulocyte-macrophage colony-stimulating factor and macrophage-colony stimulating factor receptor; all three genes may be implicated in the 5-q syndrome. A translocation between chromosomes 5 and 12, that fuses this gene to that of the translocation, ETV6, leukemia gene, results in chronic myeloproliferative disorder with eosinophiliaFor Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
| CD140b (PDGFRB) Antibody (12-1402-81) in FlowStaining of NIH/3T3 cell line with 0.5 µg of Rat IgG2a kappa Isotype Control PE(Product # 12-4321-80) (open histogram) or 0.5 µg of Anti-Mouse CD140b (PDGF Receptor b)PE (filled histogram). Total viable cells were used for analysis. |
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| 参考文献： |
| 1.Adventitial Sca1+ Cells Transduced With ETV2 Are Committed to the Endothelial Fate and Improve Vascular Remodeling After Injury."12-1402 was used in Flow cytometry/Cell sorting to provide evidence that the genetic manipulation of vascular progenitors is a promising approach to improve vascular function after endothelial injury."Authors Le Bras A,Yu B,Issa Bhaloo S,Hong X,Zhang Z,Hu Y,Xu Q2.Archivum immunologiae et therapiae experimentalisImatinib Inhibits the Renewal and Tumorigenicity of CT-26 Colon Cancer Cells after Cytoreductive Treatment with Doxorubicin."12140281 was used in flow cytometry to assess if drugs that target receptor tyrosine kinases silence or eliminate colorectal cancer cells that are refractory to conventional treatment"Authors Przybyszewska M,Miłoszewska J,Kotlarz A,Swoboda P,Pyśniak K,Szczepek W,Kaczmarek Ł,Markowicz S3.Circulation researchCardiac Fibro-Adipocyte Progenitors Express Desmosome Proteins and Preferentially Differentiate to Adipocytes Upon Deletion of the Desmoplakin Gene."12140281 was used in flow cytometry and immunocytochemistry to discuss the cellular origins of fibroadipocytes in arrhythmogenic cardiomyopathy"Authors Lombardi R,Chen SN,Ruggiero A,Gurha P,Czernuszewicz GZ,Willerson JT,Marian AJ4.Reproductive toxicology (Elmsford, N.Y.)Imatinib has deleterious effects on differentiating spermatogonia while sparing spermatogonial stem cell self renewal."12-1402 was used in Immunohistochemistry to support the possibility of utilizing germline stem cell cultures for preclinical drug tests."Authors Heim C,Minniear K,Dann CT |
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