Mouse IgG1 kappa Isotype Control, APC

货号：17-4714-41,17-4714-42

规格：25Tests,100 Tests

价格：957,1594

产品类型：小鼠同型对照

品牌：eBioscience

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：APC

抗体类型:	同型	同型对照：	IgG1, kappa
浓度：	5uL/test	用法：	Assay-Dependent (Ctrl)；Assay-Dependent (Flow)

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: APC Mouse IgG1 K Isotype Control has been reported for use in flow cytometric analysis.Applications Tested: The Mouse IgG1 K Isotype Control can be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization

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参考文献：

1. Frontiers in immunologyPhosphoproteomics Reveals Regulatory T Cell-Mediated DEF6 Dephosphorylation That Affects Cytokine Expression in Human Conventional T Cells.2017.Authors Joshi RN,Binai NA,Marabita F,Sui Z,Altman A,Heck AJR,Tegnér J,Schmidt A2. PloS onePolycyclic aromatic hydrocarbons reciprocally regulate IL-22 and IL-17 cytokines in peripheral blood mononuclear cells from both healthy and asthmatic subjects.2015.Authors Plé C,Fan Y,Ait Yahia S,Vorng H,Everaere L,Chenivesse C,Balsamelli J,Azzaoui I,de Nadai P,Wallaert B,Lazennec G,Tsicopoulos A3. PLoS pathogensExhaustion of Activated CD8 T Cells Predicts Disease Progression in Primary HIV-1 Infection.2016.Authors Hoffmann M,Pantazis N,Martin GE,Hickling S,Hurst J,Meyerowitz J,Willberg CB,Robinson N,Brown H,Fisher M,Kinloch S,Babiker A,Weber J,Nwokolo N,Fox J,Fidler S,Phillips R,Frater J4. Stem cells translational medicineEnforced Expression of HOXB4 in Human Embryonic Stem Cells Enhances the Production of Hematopoietic Progenitors but Has No Effect on the Maturation of Red Blood Cells.2016.Authors Jackson M,Ma R,Taylor AH,Axton RA,Easterbrook J,Kydonaki M,Olivier E,Marenah L,Stanley EG,Elefanty AG,Mountford JC,Forrester LM5. Journal of immunology researchThe Clinical Relevance of IL-17-Producing CD4+CD161+ Cell and Its Subpopulations in Primary Sjögren's Syndrome.2016.Authors Li L,He J,Zhu L,Yang Y,Jin Y,Jia R,Liu X,Liu Y,Sun X,Li Z

技术参数

产品应用 Ctrl;Flow

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