CD44 Monoclonal Antibody (IM7), PE-eFluor 610, eBioscience™

货号：61-0441-82

规格：100 µg

价格：3141

产品类型：流式抗体

品牌：eBioscience

抗原：cd44

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：IM7

荧光染料：PE-eFluor 610

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control (eB149/10H5), PE-eFluor 610,
免疫原性：		用法：	1 µg/test(Flow）

Description: The IM7 monoclonal antibody reacts with all isoforms of mouse CD44 (Pgp-1). CD44 is expressed by hematopoietic and non-hematopoietic cells. Bone marrow myeloid cells and memory T cells highly express this antigen and peripheral B and T cells can upregulate the expression of CD44. CD44 functions as an adhesion molecule through its binding to hyaluronate, an extracellular matrix component.Applications Reported: This IM7 antibody has been reported for use in flow cytometric analysis.Applications Tested: This IM7 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.PE-eFluor® 610 can be excited with laser lines from 488-561 nm and emits at 607 nm. We recommend using a 610/20 band pass filter (equivalent to PE-Texas Red®). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem dye is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light.Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL of cell sample + 100 µL of IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 488-561 nm; Emission: 607 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD44 cell surface antigen is a 100 kDa type 1 transmembrane glycoprotein widely expressed on human leucocytes, white matter of the brain and by some epithelial cells of the intestine and breast. Several isoforms of CD44 exist, including the predominant CD44H isoform detected in many normal tissues. CD44 is a receptor for hyaluronic acid (HA) and is involved in cell-cell interactions, cell adhesion and migration. CD44 also participates in a wide variety of cellular functions including lymphocyte activation, recirculation and homing. CD44 expression may be up-regulated upon some carcinomas, and it has been speculated that this may be related to metastatic potential. CD44 is expressed by hematopoietic, non-hematopoietic cells, epithelial tissues, and to filopodia in cultured keratinocytes. Further, bone marrow myeloid cells and memory T cells express CD44 at high levels, and peripheral B and T cells can upregulate the expression of CD44 in response to certain stimulatory events. Transcripts for the CD44 gene undergo complex alternative splicing that results in many functionally distinct isoforms, however, the full-length nature of some of these variants have not been determined. Alternative splicing is the basis for the structural and functional diversity of the CD44 protein. Diseases associated with CD44 dysfunction include superficial keratitis and lichen sclerosus. CD44 also may be related to tumor metastasis formation.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD44 Antibody (61-0441-80) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3 eFluor® 450(Product # 48-0032-82) and 0.5 µg of Rat IgG2a K Isotype Control PE-eFluor® 610(Product # 61-4321-82) (left) or 0.5 µg of Anti-Human/Mouse CD44 PE-eFluor® 610 (right).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1.TheranosticsGlutamic Pyruvate Transaminase GPT2 Promotes Tumorigenesis of Breast Cancer Cells by Activating Sonic Hedgehog Signaling.2.The Journal of clinical investigationExcessive expression of miR-27 impairs Treg-mediated immunological tolerance.3.Journal of immunology (Baltimore, Md. : 1950)The Ox40/Ox40 Ligand Pathway Promotes Pathogenic Th Cell Responses, Plasmablast Accumulation, and Lupus Nephritis in NZB/W F1 Mice.4.Nature communicationsT-B interactions are sufficient for inducing autoimmune-like chronic graft-versus-host disease.5.BMC biologyThe Z-cad dual fluorescent sensor detects dynamic changes between the epithelial and mesenchymal cellular states.

技术参数

产品应用 Flow

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