CD11b (activation epitope) Monoclonal Antibody (CBRM1/5), APC, eBioscience™

货号：17-0113-41,17-0113-42

规格：25 Tests,100 Tests

价格：1456,3256

产品类型：抗体和ELISA

品牌：eBioscience

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, APC, eBioscience™
浓度：	5 µL/Test	用法：	5 μL(0.5 μg)/test(Flow)

Description: The CBRM1/5 monoclonal antibody reacts with an activation-specific epitope of human Mac-1. CBRM1/5 binds a subset of Mac-1 molecules on neutrophils and monocytes after stimulation with chemoattractants or phorbol esters but does not recognize Mac-1 on resting myeloid cells. Through interactions with its ligands, Mac-1 participates in adhesive cell interactions. The epitope recognized by this mAb localizes to the I domain on the alpha chain of Mac-1 very close to the ligand binding site in a region that is widely exposed. CBRM1/5 blocks Mac-1 dependent adhesion to fibrinogen and ICAM-1 and inhibits chemoattractant-stimulated adhesion of eosinophils to the Intercellular Adhesion Molecule-1 (ICAM-1). It should be noted that low level activation may occur during processing of freshly drawn blood. Therefore the CBRM1.5 antibody may exhibit some binding to Mac-1 in these unstimulated samples. However, higher levels of Mac-1 expression are observed in activated samples when compared to unstimulated cells.Applications Reported: This CBRM1/5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This CBRM1/5 antibody has been pre-titrated and tested by flow cytometric analyis of stimulated peripheral blood cells. This can be used at 5 µL (0.5 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD11b (activation epitope) Antibody (17-0113-41) in Flow Staining of 5-minute PMA/Ionomycin-stimulated normal human peripheral blood cells with Mouse IgG1 kappa Isotype Control APC (Product # 17-4714-81) (blue histogram) or Anti-Human CD11b (activation epitope) APC (purple histogram).Cells in the monocyte gate were used for analysis.

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参考文献：

1. Nature communicationsLECT2 drives haematopoietic stem cell expansion and mobilization via regulating the macrophages and osteolineage cells.2016.Authors Lu XJ,Chen Q,Rong YJ,Yang GJ,Li CH,Xu NY,Yu CH,Wang HY,Zhang S,Shi YH,Chen J2. PLoS geneticsStage-dependent and locus-specific role of histone demethylase Jumonji D3 (JMJD3) in the embryonic stages of lung development.2014.Authors Li Q,Wang HY,Chepelev I,Zhu Q,Wei G,Zhao K,Wang RF3. PLoS geneticsStage-dependent and locus-specific role of histone demethylase Jumonji D3 (JMJD3) in the embryonic stages of lung development.2014.Authors Li Q,Wang HY,Chepelev I,Zhu Q,Wei G,Zhao K,Wang RF

技术参数

产品应用 Flow

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