Mouse IgG1 kappa Isotype Control, Functional Grade

货号：16-4714-82,16-4714-81,16-4714-85

规格：100μg,50μg,500μg

价格：1196,717,2177

产品类型：小鼠同型对照

品牌：eBioscience

物种：小鼠

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：其它

抗体类型:	同型对照	同型对照：
浓度：	1 mg/mL	用法：	Assay-Dependent(Ctrl); Assay-Dependent(Flow); Assay-Dependent(FN)

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: This mouse IgG1 isotype control has been reported for use in surface and intracellular flow cytometric analysis, immunohistochemcial staining, immunoprecipitation and immunoblotting (WB).Applications Tested: This Mouse IgG1 isotype control has been tested by flow cytometric analysis of human peripheral leukocytes and can be used at the same concentration as the experimental antibody.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1. Scientific reportsT cells display mitochondria hyperpolarization in human type 1 diabetes.2017.Authors Chen J,Chernatynskaya AV,Li JW,Kimbrell MR,Cassidy RJ,Perry DJ,Muir AB,Atkinson MA,Brusko TM,Mathews CE2. Molecular medicine reportsHigh mobility group box 1 induces calcification of aortic valve interstitial cells via toll-like receptor 4.2017.Authors Shen W,Zhou J,Wang C,Xu G,Wu Y,Hu Z3. OncoimmunologyPolyfunctional Melan-A-specific tumor-reactive CD8(+) T cells elicited by dacarbazine treatment before peptide-vaccination depends on AKT activation sustained by ICOS.2016.Authors Franzese O,Palermo B,Di Donna C,Sperduti I,Ferraresi V,Stabile H,Gismondi A,Santoni A,Nisticò P4. American journal of transplantation : official journal of the American Society of Transplantation and the American Society of Transplant SurgeonsSelective Sparing of Human Tregs by Pharmacologic Inhibitors of the Phosphatidylinositol 3-Kinase and MEK Pathways.2016.Authors Zwang NA,Zhang R,Germana S,Fan MY,Hastings WD,Cao A,Turka LA5. Journal of immunology (Baltimore, Md. : 1950)Unexpected Role for Adaptive αβTh17 Cells in Acute Respiratory Distress Syndrome.2015.Authors Li JT,Melton AC,Su G,Hamm DE,LaFemina M,Howard J,Fang X,Bhat S,Huynh KM,O'Kane CM,Ingram RJ,Muir RR,McAuley DF,Matthay MA,Sheppard D

技术参数

产品应用 Flow;FN;Ctrl

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