Mouse IgG1 kappa Isotype Control, Biotin

货号：13-4714-85

规格：500μg

价格：1607

产品类型：小鼠同型对照

品牌：eBioscience

物种：其它

宿主：小鼠

抗体亚型：IgG1, kappa

克隆号：P3.6.2.8.1

荧光染料：Biotin

抗体类型:	同型	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	Assay-Dependent(Ctrl)； Assay-Dependent (Flow)； Assay-Dependent(ICC)； Assay-Dependent(IF)； Assay-Dependent(IHC)

Description: The monoclonal mouse IgG1 K immunoglobulin is useful as an isotype control.Applications Reported: Biotin Mouse IgG1 K Isotype Control has been reported for use in immunocytochemistry, immunohistochemistry, and flow cytometric analysis.Applications Tested: This Mouse IgG1 Isotype Control has been tested by flow cytometric analysis of human peripheral leukocytes. It should be used at the same concentration as the experimental antibody.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1. Cancer discoveryGM-CSF Mediates Mesenchymal-Epithelial Cross-talk in Pancreatic Cancer."13-4714 was used in Flow cytometry/Cell sorting to identify and characterise a population of mesenchymal stem cells within the tumour microenvironment that promote pancreatic ductal adenocarcinoma survival and metastasis through GM-CSF signalling."AuthorsWaghray M,Yalamanchili M,Dziubinski M,Zeinali M,Erkkinen M,Yang H,Schradle KA,Urs S,Pasca Di Magliano M,Welling TH,Palmbos PL,Abel EV,Sahai V,Nagrath S,Wang L,Simeone DM2. The Journal of neuroscience : the official journal of the Society for NeurosciencePD-L1 expression by neurons nearby tumors indicates better prognosis in glioblastoma patients."13-4714 was used in Immunofluorescence to study the relationship between program death-ligand expression and clinical outcome in glioblastoma multiforme patients."AuthorsLiu Y,Carlsson R,Ambjørn M,Hasan M,Badn W,Darabi A,Siesjö P,Issazadeh-Navikas S

技术参数

产品应用 Ctrl;IHC;ICC;IF;Flow

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