CD11b Monoclonal Antibody (ICRF44), eBioscience™

货号：14-0118-82

规格：100μg

价格：1181

产品类型：流式抗体

品牌：eBioscience

抗原：CD11b

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：ICRF44

荧光染料：其它

抗体类型:	单抗	同型对照：	IgG1, kappa
浓度：	0.5 mg/mL	用法：	1 µg/test (Flow)；Assay-Dependent(FN)； Assay-Dependent(IHC (F))；Assay-Dependent(Neu)

Description: The ICRF44 monoclonal antibody reacts with human CD11b, a 165 kDa adhesion molecule. CD11b associated with integrin beta2 (CD18) is expressed on the surface of monocytes, granulocytes, activated lymphocytes and a subset of NK cells. CD11b is a receptor for intercellular adhesion molecule family members CD54, CD102 and CD50 as well as for iC3b. These adhesions are crucial in cell-cell and cell-matrix interactions.Applications Reported: The ICRF44 antibody has been reported for use in flow cytometric analysis, and immunohistochemical staining on formaldehyde fixed tissue. ICRF44 has also been reported in inhibition of aggregation of PMNs.Applications Tested: The ICRF44 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Purity: Greater than 90%, as determined by SDS-PAGE.Aggregation: Less than 10%, as determined by HPLC.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD11b (integrin alpha-M, ITGAM, integrin alpha-X, ITGAX) is a 165 kDa adhesion molecule that associates non-covalently with integrin beta-2 (CD18). The CD11b/CD18 heterodimeric complex is also known as integrin alpha-M beta-2, Mac-1, and CR3 (complement receptor 3). CD11b is expressed on the surface of monocytes/macrophages, granulocytes, activated lymphocytes, a subset of NK cells, a subset of dendritic cells, and microglia in the brain. CD11b/CD18 functions as the receptor for ICAM-1 (CD54), ICAM-2 (CD102), ICAM-4 (CD242), CD14, CD50, CD23, heparin, iC3b, fibrinogen, and Factor X -these adhesions are critical for cell-cell and cell-matrix interactions. CD11b is expressed on 8% of spleen cells, 44% of bone marrow cells, and less than 1% of thymocytes, and is commonly used as a microglial marker in nervous tissue. The expression of CD11b increases during monocyte maturation and expression levels vary on tissue macrophages. Further, peritoneal macrophages are reported to express higher levels of CD11b than splenic macrophages. Diseases associated with CD11b dysfunction include systemic lupus erythematosus 6 and ITGAM-related susceptibility to systemic lupus erythematosus.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD11b Antibody (14-0118-82) in FlowStaining of normal human peripheral blood cells with Anti-Human CD11b PE. Autofluorescence is indicatedby open histogram. Cells in the monocyte population were used for analysis.

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参考文献：

1. Nature communicationsCD19 CAR immune pressure induces B-precursor acute lymphoblastic leukaemia lineage switch exposing inherent leukaemic plasticity.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. OncotargetThe prognostic effects of tumor infiltrating regulatory T cells and myeloid derived suppressor cells assessed by multicolor flow cytometry in gastric cancer patients."14-0118 was used in Flow cytometry/Cell sorting to demonstrate that in gastric cancer, a high frequency of Tregs among tumour-infiltrating CD4+ T cells is favourable, but an increased proportion of MDSCs is an adverse independent prognostic factor."AuthorsChoi HS,Ha SY,Kim HM,Ahn SM,Kang MS,Kim KM,Choi MG,Lee JH,Sohn TS,Bae JM,Kim S,Kang ES3. Nature communicationsdNP2 is a blood-brain barrier-permeable peptide enabling ctCTLA-4 protein delivery to ameliorate experimental autoimmune encephalomyelitis....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. Experimental hematologyInduction of autophagy is a key component of all-trans-retinoic acid-induced differentiation in leukemia cells and a potential target for pharmacologic modulation....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. Journal of neuropathology and experimental neurologyActivation status of human microglia is dependent on lesion formation stage and remyelination in multiple sclerosis."14-0118 was used in Flow cytometry/Cell sorting to define the differential activation states of microglia during lesion development in multiple sclerosis-affected CNS tissues."AuthorsPeferoen LA,Vogel DY,Ummenthum K,Breur M,Heijnen PD,Gerritsen WH,Peferoen-Baert RM,van der Valk P,Dijkstra CD,Amor S

技术参数

产品应用 Flow;FN;Neu

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