CD18 (LFA-1 beta) Monoclonal Antibody (M18/2), FITC, eBioscience™

货号：11-0181-81 ,11-0181-82 ,11-0181-85

规格：50 µg,100 µg,500 µg

价格：0,1545,3925

产品类型：流式抗体

品牌：eBioscience

抗原：CD18

物种：小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：M18/2

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	1 µg/test (Flow）

产品详细信息Description: The M18/2 monoclonal antibody reacts with mouse CD18, the 90-95 kDa integrin beta 2 glycoprotein. CD18 is the common beta subunit that non-covalently associates with CD11a (alpha L), CD11b (alpha M) and CD11c (alpha X) to form LFA-1, Mac-1 and gp150/95. Heterodimers of CD18 and the a subunits are expressed broadly by all leukocytes with NK and T cells showing higher density of surface expression. Through the interactions of LFA-1, Mac-1 and gp150/95 with their counter-receptors, CD18 is involved in cellular adhesion and the immune response.Applications Reported: The M18/2 antibody has been reported for use in flow cytometric analysis.Applications Tested: The M18/2 antibody has been tested by flow cytometric analysis of mouse splenocytes and bone marrow cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD18 integrin beta 2 subunit is a 90 kDa type I transmembrane protein expressed on all leucocytes. CD18 can combine with integrin molecules CD11a-c to form heterodimers at the cell surface, and these heterodimers are known to participate in the process of cell adhesion as well as cell-surface mediated signaling. CD18 forms heterodimers with four types of CD11 molecule to constitute leukocyte (beta2) integrins: alphaLbeta2 (CD11a/CD18, LFA-1), alphaMbeta2 (CD11b/CD18, Mac-1, CR3), alphaXbeta2 (CD11c/CD18) and alphaDbeta2 (CD11d/CD18). In most cases, the response mediated by the integrin is a composite of the functions of its individual subunits, and these integrins are essential for proper leukocyte migration, mediating intercellular contacts. Absence of CD18 leads to leukocyte adhesion deficiency-1, severe reduction of CD18 expression leads to the development of a psoriasiform skin disease. CD18 is also a target of Mannheimia (Pasteurella) haemolytica leukotoxin and is sufficient to mediate leukotoxin-mediated cytolysis. Defects in the CD18 gene are the cause of leukocyte adhesion deficiency type I (LAD1). Two transcript variants encoding the CD18 protein have been identified.

数据：

CD18 (LFA-1 beta) Antibody (11-0181-81)Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas 2012 -Published figure using CD18 (LFA-1 beta) monoclonal antibody (Product # 11-0181-81)

推荐产品：

▪ 流抗抗体

参考文献：

1.Biochimica et biophysica acta Antigen presenting cell abnormalities in the Cln3(-/-) mouse model of juvenile neuronal ceroid lipofuscinosis. 2.PloS one Monoacylglycerol lipase (MAGL) inhibition attenuates acute lung injury in mice. 3.The Journal of infectious diseases Glutaminyl cyclases as novel targets for the treatment of septic arthritis. 4.Brazilian journal of medical and biological research = Revista brasileira de pesquisas medicas e biologicas Impairment of the hematological response and interleukin-1β production in protein-energy malnourished mice after endotoxemia with lipopolysaccharide. 5.The Journal of experimental medicine Dependence of proliferative vascular smooth muscle cells on CD98hc (4F2hc, SLC3A2).

技术参数

产品应用 Flow

© Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：https://biorod.cn/thread-16945.htm