CD16 Monoclonal Antibody (eBioCB16 (CB16)), FITC, eBioscience™

货号：11-0168-41,11-0168-42

规格：25 Tests,100 Tests

价格：867,2342

产品类型：流式抗体

品牌：eBioscience

抗原：CD16

物种：人

宿主：小鼠

抗体亚型：其它

克隆号：eBioCB16 (CB16)

荧光染料：FITC

抗体类型:	流式抗体	同型对照：	Mouse IgG1 kappa Isotype Control (P3.6.2.8.1), FITC, eBioscience™
浓度：	5 µL/Test	用法：	5uL（0.125µg）/test(Flow）

产品详细信息Description: The eBioCB16 monoclonal antibody recognizes CD16 (Fc gammaRIII), the low-affinity receptor for IgG with an apparent molecular weight of 50-80 kDa. CD16 is represented by two similar genes, CD16A (Fc gammaRIIIA), which exists as a hetero-oligomeric polypeptide-anchored form in macrophages and NK cells and CD16B (Fc gammaRIIIB), which exist as a monomeric GPI-anchored form in neutrophils. Furthermore, there are two known polymorphisms of CD16B, NA-1 and NA-2. Individuals homozygous for NA-2 show a lower phagocytic capacity compared with NA-1. CD16 binds IgG in the form of immune complexes and shows preferential binding of IgG1 and IgG3 isotypes and minimal binding of IgG2 and IgG4. Upon IgG binding, both CD16 isoforms initiate signal transduction cascades that lead to a variety of responses including antibody-dependent cell-mediated cytotoxicity (ADCC), phagocytosis, degranulation and proliferation.Applications Reported: This eBioCB16 (CB16) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioCB16 (CB16) antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered. 靶标信息CD16 (FCGR3A) is a 50-65 kDa cell surface molecule that exists in two forms - a transmembranous form expressed by NK cells and some T cells, and a phosphatidylinositol linked form expressed by granulocytes. CD16 is a low affinity receptor for IgG (FcR III), and is an important receptor mediating ADCC by NK cells. Human CD16 is expressed in two forms – FCGR3A and FCGR3B. FCGR3A is associated with the FcepsilonRI-gamma subunit and is responsible for antibody-dependent NK cell cytotoxicity. Mast cell FCGR3A is associated, with FcepsilonRI-beta subunit. Besides IgG, FCGR3A can be triggered also by oligomeric IgE. FCGR3B is a GPI-linked monomeric receptor expressed on neutrophils and is involved in their activation and induction of a pro-adhesive phenotype. Diseases associated with CD16 dysfunction include immunodeficiency 20 and systemic lupus erythematosus.

数据

CD16 Antibody (11-0168-41) in FlowStaining of normal human peripheral blood cells with Anti-Human CD56 (NCAM) PE(Product # 12-0567-42) and Mouse IgG1 K Isotype Control FITC (Product # 11-4714-42)(left) or Anti-Human CD16 FITC (right). Cells in the lymphocyte gate were used for analysis. CD16 Antibody (11-0168-41) Staining of human peripheral blood cells. As expected based on known relative expression patterns, CD16 clone CB16 stains all granulocytes, a subset of monocytes and a subset of lymphocytes (NK cells). Details: Normal human whole blood was surface stained with CD16 (clone CB16). After staining, red blood cells were lysed using 1-step Fix/Lyse Buffer. Cells in the lymphocyte (purple histogram), monocyte (orange histogram), or granulocyte (blue histogram) gates were used for analysis.

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参考文献：

1.Nature communications A child with perinatal HIV infection and long-term sustained virological control following antiretroviral treatment cessation.2.Nature communications Bioreactor-based mass production of human iPSC-derived macrophages enables immunotherapies against bacterial airway infections.3.JCI insight NF-κB/MAPK activation underlies ACVR1-mediated inflammation in human heterotopic ossification.4.Oncotarget Molecular signatures associated with tumor-specific immune response in melanoma patients treated with dendritic cell-based immunotherapy.5.OncoTargets and therapy A DNA vaccine encoding mutated HPV58 mE6E7-Fc-GPI fusion antigen and GM-CSF and B7.1.

技术参数

产品应用 Flow；IF

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