CD36 Monoclonal Antibody (eBioNL07 (NL07)), eFluor 660

货号：50-0369-42,50-0369-41

规格：100 Tests,25 Tests

价格：2677,0

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgM

克隆号：eBioNL07 (NL07)

荧光染料：eFluor 660

抗体类型:	单抗	同型对照：	Mouse IgM Isotype Control, eFluor 660, eBioscience™
免疫原性：		用法：	5uL（0.125µg）/test(Flow）

Description: The monoclonal antibody eBioNL07 recognizes human CD36, which is a member of the class B scavenger receptor family. CD36 was originally identified as a platelet-membrane glycoprotein also called glycoprotein IV and a receptor for thrombospondin-1 (TSP-1) and extracellular matrix proteins. Binding to TSP-1 is in the CLESH (CD36 LIMP-II Emp sequence homology) domain of CD36. CD36 expression is broad and includes microvascular (but not large vessel) endothelium, adipocytes, skeletal muscle, dendritic cells, epithelia of the retina, breast, and intestine, smooth muscle cells, and hematopoietic cells, including erythroid precursors, platelets, monocytes/macrophages, DCs and megakaryocytes. Expression on platelets is absent on Nak-a negative donors. Unlike other scavenger receptor, CD36 binds LDL that has been exposed to "minimally" oxidizing conditions. CD36 is also a fatty acid translocase (FAT) necessary for the transport of long-chain fatty acids (LCFAs) and therefore may play a role in atherosclerosis.Applications Reported: This eBioNL07 (NL07) antibody has been reported for use in flow cytometric analysis.Applications Tested: This eBioNL07 (NL07) antibody has been pre-titrated and tested by flow cytometric analysis of normal human perioheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD36 (fatty acid translocase, FAT) is an 88 kDa, ditopic glycosylated protein that belongs to the class B family of scavenger receptors. CD36 is expressed by most resting marginal zone B cells but not by follicular and B1 B cells, and is rapidly induced on Follicular B cells in vitro upon TLR and CD40 stimulation. CD36 does not affect the development of B cells, but modulates both primary and secondary antibody response. Similar to glucose transporter GLUT4, CD36 is translocated from intracellular pools to the plasma membrane following cell stimulation by insulin. In mouse, CD36 is responsible for gustatory perception of long-chain fatty acids. CD36 is preferentially found within lipid rafts, which facilitates its association with receptors, signaling and adapter molecules. CD36 binds to collagen, thrombospondin, anionic phospholipids and oxidized LDL. Further, CD36 may function as a cell adhesion molecule and directly mediates cyto-adherence of Plasmodium falciparum parasitized erythrocytes. Mutations in the CD36 gene cause platelet glycoprotein deficiency. Multiple alternatively spliced transcript variants encoding the CD36 protein have been found.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.CD36 Antibody (50-0369-42) in FlowStaining of normal human peripheral blood cells with Mouse IgM Isotype Control eFluor® 660 (Product # 50-4752-82) (blue histogram) or Anti-Human CD36 eFluor® 660 (purple histogram). Cells in the monocyte gate were used for analysis.

数据

CD36 Antibody (50-0369-42) in Flow Staining of normal human peripheral blood cells with Mouse IgM Isotype Control eFluor® 660 (Product # 50-4752-82) (blue histogram) or Anti-Human CD36 eFluor® 660 (purple histogram). Cells in the monocyte gate were used for analysis.

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参考文献：

1. Scientific reportsIRGM1 regulates oxidized LDL uptake by macrophage via actin-dependent receptor internalization during atherosclerosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Stem cell researchHeterogeneity and immunophenotypic plasticity of malignant cells in human liposarcomas."50-0369 was used in Flow cytometry/Cell sorting to show that malignant liposarcoma cells in WDLS and DDLS can be classified according to distinct stages of adipogenesis."AuthorsZhang Y,Young ED,Bill K,Belousov R,Peng T,Lazar AJ,Pollock RE,Simmons PJ,Lev D,Kolonin MG3. OncogeneTerminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. OncogeneTerminal differentiation and loss of tumorigenicity of human cancers via pluripotency-based reprogramming.By clicking the link above, you will be redirected to the Benchsci website.Disclaime

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