CD24 Monoclonal Antibody (M1/69), FITC, eBioscience™

货号：11-0242-81,11-0242-82,11-0242-85

规格：50 µg,100 µg,500 µg

价格：752,1280,2886

产品类型：流式抗体

品牌：eBioscience

抗原：CD24

物种：小鼠

宿主：大鼠

抗体亚型：IgG2b, kappa

克隆号：M1/69

荧光染料：FITC

类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, FITC, eBioscience™
浓度：	0.5 mg/mL	用法：	0.5 µg/test(Flow）

产品详细信息Description: The M1/69 monoclonal antibody reacts with the mouse CD24 molecule, also known as Heat Stable Antigen (HSA). This 35-50 kDa molecule is anchored in the plasma membrane via phosphatidylinositol and is expressed by erythrocytes, thymocytes, peripheral lymphocytes and myeloid lineage. CD24 is a variably glycosylated molecule resulting in heterogeneity of molecular mass of this antigen on cells of different lineages and antibodies to CD24 exhibit subtle differences in staining level on lymphocyte populations. The expression of CD24 has been used to resolve stages of B lymphopoiesis in mouse bone marrow. It has been reported that P-selectin (CD62P) binds to CD24.Applications Reported: The M1/69 antibody has been reported for use in flow cytometric analysis.Applications Tested: The M1/69 antibody has been tested by flow cytometric analysis of mouse splenocytes and thymocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 488 nm; Emission: 520 nm; Laser: Blue Laser.Filtration: 0.2 µm post-manufacturing filtered.靶标信息CD24 is a two-chain glycosylphosphatidylinositol (GPI)-anchored glycoprotein expressed at multiple stages of B-cell development, beginning with the bone marrow pro-B-cell compartment and continuing through mature, surface Ig positive B-cells. Plasma cell expression is very low or negative. CD24 is also expressed on the majority of B-lineage acute lymphoblastic leukemias, B-cell CCLs and B-cell non-Hodgkin's lymphomas. CD24 may play a role in regulation of B-cell proliferation and maturation, andcontrol of autoimmunity.

数据

CD24 Antibody (11-0242-82) in FlowStaining of C57BL/6 splenocytes with Anti-Mouse CD3e APC (Product # 17-0031-82)and 0.25 µg of Rat IgG2b K Isotype Control FITC (Product # 11-4031-82) (left) or0.25 µg of Anti-Mouse CD24 FITC (right). Cells in the lymphocyte gatewere used for analysis.

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参考文献：

1.PloS oneSevere Changes in Thymic Microenvironment in a Chronic Experimental Model of Paracoccidioidomycosis."11024282 was used in flow cytometry to examine the thymic microenvironment using a model of paracoccidioidomycosis"AuthorsAlves da Costa T,Di Gangi R,Thomé R,Barreto Felisbino M,Pires Bonfanti A,Lumi Watanabe Ishikawa L,Sartori A,Burger E,Verinaud L 2.Scientific reportsCD24 induces changes to the surface receptors of B cell microvesicles with variable effects on their RNA and protein cargo."11-0242 was used in Flow cytometry/Cell sorting to perform a systematic characterisation of B cell microvesicles released from WEHI-231 B lymphoma cells in response to CD24 stimulation."AuthorsAyre DC,Chute IC,Joy AP,Barnett DA,Hogan AM,Grüll MP,Peña-Castillo L,Lang AS,Lewis SM,Christian SL3.CellEpithelia Use Butyrophilin-like Molecules to Shape Organ-Specific γδ T Cell Compartments."11-0242 was used in Flow cytometry/Cell sorting to investigate the mechanism that shape organ-specific γδ T cell compartments, showing that Btnl1 expressed by murine enterocytes shapes the local γδ compartment, as does BTNL3 and BTNL8 expressed by human gut epithelial cells, suggesting a conserved mechanism shaped local T cell compartments."AuthorsDi Marco Barros R,Roberts NA,Dart RJ,Vantourout P,Jandke A,Nussbaumer O,Deban L,Cipolat S,Hart R,Iannitto ML,Laing A,Spencer-Dene B,East P,Gibbons D,Irving PM,Pereira P,Steinhoff U,Hayday A4.The Journal of experimental medicineNKT sublineage specification and survival requires the ubiquitin-modifying enzyme TNFAIP3/A20."11-0242 was used in Flow cytometry/Cell sorting to show that negative regulation of TCR signalling during NKT development controls the differentiation and survival of NKT1 and NKT2 cells."AuthorsDrennan MB,Govindarajan S,Verheugen E,Coquet JM,Staal J,McGuire C,Taghon T,Leclercq G,Beyaert R,van Loo G,Lambrecht BN,Elewaut D5.The Journal of biological chemistryThe Transcriptional Repressor ZNF503/Zeppo2 Promotes Mammary Epithelial Cell Proliferation and Enhances Cell Invasion.

技术参数

产品应用 Flow；IF

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