CD45R (B220) Monoclonal Antibody (RA3-6B2), APC-eFluor 780

货号：47-0452-82,47-0452-80

规格：100μg,25μg

价格：1817,925

产品类型：流式抗体

品牌：Thermo Fisher

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：RA3-6B2

荧光染料：APC-eFluor 780

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, APC-eFluor 780, eBioscience™
免疫原性：		用法：	0.5 µg/test(Flow）

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.5 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD45R (B220) Antibody (47-0452-82) in Flow Staining of C57BL/6 splenocytes with Anti-Mouse CD3e FITC (Product # 11-0031-82) and 0.25 µg of Rat IgG2a kappa Isotype Control APC-eFluor® 780 (Product # 47-4321-82) (left) or 0.25 µg of Anti-Human/Mouse CD45R (B220)APC-eFluor® 780 (right). Total viable cells were used for analysis.

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参考文献：

1. The Journal of experimental medicineEpithelial Fli1 deficiency drives systemic autoimmunity and fibrosis: Possible roles in scleroderma.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Nature cell biologyDifferential cytokine contributions of perivascular haematopoietic stem cell niches.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. Journal of immunology (Baltimore, Md. : 1950)Autophagy in Dendritic Cells and B Cells Is Critical for the Inflammatory State of TLR7-Mediated Autoimmunity."47045282 was used in flow cytometry to analyze the effects of a loss of autophagy in dendritic cells and B cells in a TLR7-mediated model of autoimmunity"AuthorsWeindel CG,Richey LJ,Mehta AJ,Shah M,Huber BT4. PloS oneDifferential regulation of C5a receptor 1 in innate immune cells during the allergic asthma effector phase."47-0452 was used in Flow cytometry/Cell sorting to demonstrate a complex regulation pattern of C5aR1 in the airways, lung tissue and lymph nodes of mice."AuthorsEnder F,Wiese AV,Schmudde I,Sun J,Vollbrandt T,König P,Laumonnier Y,Köhl J5. PloS oneAcute and chronic effects of treatment with mesenchymal stromal cells on LPS-induced pulmonary inflammation, emphysema and atherosclerosis development."47-0452 was used in Flow cytometry/Cell sorting to investigate the immunomodulatory properties of MSC in acute and chronic models of lipopolysaccharide (LPS)-induced inflammation, emphysema and atherosclerosis development."AuthorsKhedoe PPSJ,de Kleijn S,van Oeveren-Rietdijk AM,Plomp JJ,de Boer HC,van Pel M,Rensen PCN,Berbée JFP,Hiemstra PS

技术参数

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