CD4 Monoclonal Antibody (GK1.5), APC-eFluor 780

货号：47-0041-80,47-0041-82

规格：25μg,100μg

价格：822,2122

产品类型：流式抗体

品牌：Thermo Fisher

物种：小鼠

宿主：大鼠

抗体亚型：IgG

克隆号：GK1.5

荧光染料：APC-eFluor 780

抗体类型:	单抗	同型对照：	Rat IgG2b kappa Isotype Control, APC-eFluor 780, eBioscience™
免疫原性：		用法：	0.125 µg/test(Flow）

Description: The GK1.5 monoclonal antibody reacts with the mouse CD4 molecule, a 55 kDa cell surface receptor expressed by a majority of thymocytes, subpopulation of mature T cells and dendritic cells. CD4 binds to MHC class II on the surface of antigen presenting cells and plays an important role both in T cell development and in optimal functioning of mature T cells. In T cells, CD4 associates with protein tyrosine kinase p56lck through its cytoplasmic tail. Binding of GK1.5 is blocked by RM4-5.Applications Reported: This GK1.5 antibody has been reported for use in flow cytometric analysis.Applications Tested: This GK1.5 antibody has been tested by flow cytometric analysis of mouse splenocytes. This can be used at less than or equal to 0.125 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.APC-eFluor 780 emits at 780 nm and is excited with the Red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Light sensitivity: This tandem is sensitive to photo-induced oxidation. Please protect this vial and stained samples from light. Fixation: Samples can be stored in IC Fixation Buffer (cat. 00-8222) (100 µL cell sample + 100 µL IC Fixation Buffer) or 1-step Fix/Lyse Solution (cat. 00-5333) for up to 3 days in the dark at 4°C with minimal impact on brightness and FRET efficiency/compensation. Some generalizations regarding fluorophore performance after fixation can be made, but clone specific performance should be determined empirically.Excitation: 633-647 nm; Emission: 780 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe CD4 antigen is involved in the recognition of MHC class II molecules and is a co-receptor for HIV. CD4 is primarily expressed in a subset of T-lymphocytes, also referred to as T helper cells, but may also be expressed by other cells in the immune system, such as monocytes, macrophages, and dendritic cells. At the tissue level, CD4 expression may be detected in thymus, lymph nodes, tonsils, and spleen, and also in specific regions of the brain, gut, and other non-lymphoid tissues. CD4 functions to initiate or augment the early phase of T-cell activation through its association with the T-cell receptor complex and protein tyrosine kinase, Lck. It may also function as an important mediator of direct neuronal damage in infectious and immune-mediated diseases of the central nervous system. Multiple alternatively spliced transcripts have been identified in this gene [RefSeq, July 2017].For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

CD4 Antibody (47-0041-80) in Flow Staining of C57Bl/6 splenocytes with Anti-Mouse CD19 FITC (Product # 11-0193-82) and 0.06 µg of Rat IgG2b K Isotype Control APC-eFluor® 780 (Product # 47-4031-82) (left) or 0.06 µg of Anti-Mouse CD4 APC-eFluor® 780 (right).Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Stem cell reportsMSC-derived Extracellular Vesicles Attenuate Immune Responses in Two Autoimmune Murine Models: Type 1 Diabetes and Uveoretinitis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. Immunity, inflammation and diseaseLocation of tumor affects local and distant immune cell type and number.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. RNA biologyADAR1 deletion induces NFκB and interferon signaling dependent liver inflammation and fibrosis.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer3. JCI insightIrgm1 coordinately regulates autoimmunity and host defense at select mucosal surfaces.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer4. OncoimmunologyCompensatory upregulation of PD-1, LAG-3, and CTLA-4 limits the efficacy of single-agent checkpoint blockade in metastatic ovarian cancer.... By clicking the link above, you will be redirected to the Benchsci website.Disclaimer5. European journal of medical researchThe inflammatory phase of fracture healing is influenced by oestrogen status in mice."47-0041 was used in Immunohistochemistry to indicate that oestrogen-deficiency influences the early inflammatory phase after fracture."AuthorsHaffner-Luntzer M,Fischer V,Prystaz K,Liedert A,Ignatius A

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