Mouse IgG2b kappa Isotype Control, APC
货号：17-4732-81
规格：50μg
价格：1132
产品类型：小鼠同型对照
品牌：Thermo Fisher
物种：其它
宿主：小鼠
抗体亚型：IgG2b, kappa
克隆号：eBMG2b
荧光染料：APC
抗体类型: | 单抗 | 同型对照： | IgG2b, kappa |
免疫原性： | | 用法： | Assay-Dependent (Ctrl)；Assay-Dependent (Flow) |
Description: The monoclonal mouse IgG2b, kappa is useful as an isotype control immunoglobulin.Applications Reported: APC Mouse IgG2b Iso Cntrl has been reported for use in flow cytometric analysis.Applications Tested: APC Mouse IgG2b Isotype Control has been tested by flow cytometric analysis of normal human peripheral blood cells. This should be used at the same concentration as the experimental antibody.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationThe isotype of a primary antibody and the application it is being used in can result in background staining. Primary antibody background noise can be caused by binding to Fc receptors on target cells; by non-specific interactions with cellular proteins, carbohydrates, and lipids; or by cell autofluorescence. Isotype control antibodies can act as negative controls to help differentiate non-specific background signal from specific antibody signal because they have no relevant specificity to a target antigen. While isotype controls are most commonly used in flow cytometry, they are useful in other applications such as chromatin immunoprecipitation (ChIP), immunohistochemistry, and gel shifts. Isotype controls should match with the primary antibody species and isotype so that the level of specific staining by the primary antibody may be accurately determined. If using directly labeled primary antibodies, the isotype control works best if conjugated with the same label as the test antibody.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
推荐产品： |
| 流式抗体细胞周期检测细胞凋亡检测细胞增殖活性检测 |
参考文献： |
| 1.Journal of immunology (Baltimore, Md. : 1950) Soluble CD80 restores T cell activation and overcomes tumor cell programmed death ligand 1-mediated immune suppression. "17-4732 was used in Blocking experiments to identify CD80-Fc as an alternative and potentially more efficacious therapeutic agent for overcoming PDL1-induced immune suppression and facilitating tumor-specific immunity." AuthorsHaile ST,Dalal SP,Clements V,Tamada K,Ostrand-Rosenberg S |
©
Bio-Rad伯乐代理官网-伯乐抗体-伯乐层析Aminex色谱柱是专业的授权总代理区域代理经销平台。
© 如需询价，请加客服QQ：1749072012 、客服微信：jinshanbio，或发送邮件到1749072012@qq.com
© 平台为生命科学研究相关领域提供一站式耗材试剂仪器解决方案和采购服务，数据资源基于CC协议。
© 本文地址：
https://biorod.cn/thread-17037.htm
QQ:1749072012