CD28 Monoclonal Antibody (CD28.2), Functional Grade功能抗体

货号：16-0289-81,16-0289-85,16-0289-38,16-0289-025,16-0289-050

规格：50μg,500μg,5mg,25mg,50mg

价格：1283,3767,24089,72266,120443

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：小鼠

抗体亚型：IgG

克隆号：CD28.2

荧光染料：其它

抗体类型:	单抗	同型对照：	Mouse IgG1 kappa Isotype Control, Functional Grade, eBioscience™
免疫原性：		用法：	1 μg/test(Flow)；Assay-Dependent (FN)

Description: The CD28.2 monoclonal antibody reacts with the human CD28 molecule, a 44 kDa homodimer expressed by thymocytes, mature T cells and plasma cells. CD28 is a ligand for CD80 (B7-1) and CD86 (B7-2) and is a potent co-stimulator of T cells. Signaling through CD28 augments IL-2 and IL-2 receptor expression as well as cytotoxicity of CD3-activated T cells.Applications Reported: The CD28.2 antibody has been reported for use in flow cytometric analysis. CD28.2 has also been reported in costimulation of T cells in in vitro functional assays.Applications Tested: TheCD28.2 antibody has been tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Endotoxin: Less than 0.001 ng/ug antibody as determined by the LAL assay.Storage and handling: Use in a sterile environment.Filtration: 0.2 µm post-manufacturing filtered.Purity: Greater than 90%, as determined by SDS-PAGE.Endotoxin Level: Less than 0.001 ng/µg antibody, as determined by LAL assay.Aggregation: Less than 10%, as determined by HPLC.Background/Target InformationCD28 antigen is a 44 kDa disulfide linked homodimeric T cell specific surface glycoprotein. CD28 is a cell adhesion molecule of the immunoglobulin superfamily which is constitutively expressed on most peripheral blood T lymphocytes. Moreover, CD28 is the critical T cell costimulatory receptor that provides the cell the important second activation signal by binding CD80 and CD86 which are expressed by antigen presenting cells. In addition to its co-stimulation role, CD28 functions by preventing T cells from entering an anergic-hyporesponsive state or from undergoing premature apoptotic cell death. In murine peripheral lymphoid organs and in the blood, all CD4+ and CD8+ T cells express CD28. In the thymus, CD28 expression is highest on immature CD3-, CD8+ and CD4+8+ cells, and on CD4-8- cells that express alpha/beta and gamma/delta TCR. The level of CD28 on mature CD4+ and CD8+ alpha/beta TCR+ thymocytes is two- to fourfold lower than on the immature cells. Diseases associated with CD28 dysfunction include mycosis fungiodes and Sezary's Disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1. Cell death and diseaseRANKL-mediated harmonious dialogue between fetus and mother guarantees smooth gestation by inducing decidual M2 macrophage polarization."16-0289 was used in Functional assays to investigate the effect of RANKL from human embryonic trophoblasts and maternal decidual stromal cells on decidual macrophage differentiation and maternal-fetal immune tolerance."AuthorsMeng YH,Zhou WJ,Jin LP,Liu LB,Chang KK,Mei J,Li H,Wang J,Li DJ,Li MQ2. Frontiers in microbiologyA Novel Bromodomain Inhibitor Reverses HIV-1 Latency through Specific Binding with BRD4 to Promote Tat and P-TEFb Association."16-0289 was used in Functional assays to evaluate the potency of UMB-32 based methyl-triazolo bromodomain, and extra-terminal domain inhibitors as potential new latency-reversing agents."AuthorsHuang H,Liu S,Jean M,Simpson S,Huang H,Merkley M,Hayashi T,Kong W,Rodríguez-Sánchez I,Zhang X,Yosief HO,Miao H,Que J,Kobie JJ,Bradner J,Santoso NG,Zhang W,Zhu J3. The Journal of allergy and clinical immunologyImmune dysregulation in patients with PTEN hamartoma tumor syndrome: Analysis of FOXP3 regulatory Tcells."16-0289 was used in Functional assays to investigate Treg cells in patients with heterozygous germline PTEN mutations, showing a significant effect on T- and B-cell immunity."AuthorsChen HH,Händel N,Ngeow J,Muller J,Hühn M,Yang HT,Heindl M,Berbers RM,Hegazy AN,Kionke J,Yehia L,Sack U,Bläser F,Rensing-Ehl A,Reifenberger J,Keith J,Travis S,Merkenschlager A,Kiess W,Wittekind C,Walker L,Ehl S,Aretz S,Dustin ML,Eng C,Powrie F,Uhlig HH4. Molecular biology of the cellClus-DoC: a combined cluster detection and colocalization analysis for single-molecule localization microscopy data."16-0289 was used in Functional assays to develop Clus-DoC, an analysis strategy that quantifies both the spatial distribution of a protein and its colocalization status."AuthorsPageon SV,Nicovich PR,Mollazade M,Tabarin T,Gaus K5. Proceedings of the National Academy of Sciences of the United States of AmericaFunctional role of T-cell receptor nanoclusters in signal initiation and antigen discrimination."16-0289 was used in Functional assays to investigate how TCR-CD3 clusters can translate antigen recognition into signal initiation, and how cluster formation is a process of antigen discrimination."AuthorsPageon SV,Tabarin T,Yamamoto Y,Ma Y,Nicovich PR,Bridgeman JS,Cohnen A,Benzing C,Gao Y,Crowther MD,Tungatt K,Dolton G,Sewell AK,Price DA,Acuto O,Parton RG,Gooding JJ,Rossy J,Rossjohn J,Gaus K

技术参数

产品应用 Flow;FN

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