CD45R (B220) Monoclonal Antibody (RA3-6B2), APC

货号：17-0452-81,17-0452-82,17-0452-83

规格：50μg,100μg,200μg

价格：1518,2534,3551

产品类型：流式抗体

品牌：Thermo Fisher

物种：人

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：RA3-6B2

荧光染料：APC

抗体类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control, APC, eBioscience™
免疫原性：		用法：	0.25 µg/test(Flow）

Description: The RA3-6B2 monoclonal antibody reacts with exon A-restricted isoform of mouse CD45, a 220 kDa surface molecule. CD45R/B220 epitope is mainly expressed by the B cell lineage from early Pro-B to mature B cells. However, some activated T cells, lymphokine activated killer cells (LAK), NK cell progenitors in the bone marrow, and T cells of the lpr/lpr mutant mouse also express this antigen.Applications Reported: This RA3-6B2 antibody has been reported for use in flow cytometric analysis.Applications Tested: This RA3-6B2 antibody has been tested by flow cytometric analysis of mouse spleen cells. This can be used at less than or equal to 0.25 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.Excitation: 633-647 nm; Emission: 660 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD45R is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. CD45R contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains. It is specifically expressed in hematopoietic cells and has been shown to be an essential regulator of T- and B-cell antigen receptor signaling.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

CD45R (B220) Antibody (17-0452-81) in FlowStaining of C57Bl/6 splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Human/Mouse CD45R (B220) APC (right). Cells in the lymphocyte gate were used for analysis.

数据

CD45R (B220) Antibody (17-0452-81) in Flow Staining of C57Bl/6 splenocytes with Anti-Mouse CD3 PerCP-eFluor® 710 (Product # 46-0032-82) and 0.125 µg of Rat IgG2a K Isotype Control APC (Product # 17-4321-81) (left) or 0.125 µg of Anti-Human/Mouse CD45R (B220) APC (right). Cells in the lymphocyte gate were used for analysis.

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参考文献：

1. Frontiers in immunologyProapoptotic BIM Impacts B Lymphoid Homeostasis by Limiting the Survival of Mature B Cells in a Cell-Autonomous Manner.By clicking the link above, you will be redirected to the Benchsci website.Disclaimer2. OncoimmunologyThe Interleukin (IL)-1R1 pathway is a critical negative regulator of PyMT-mediated mammary tumorigenesis and pulmonary metastasis."17-0452 was used in Flow cytometry/Cell sorting to genetically investigate the role of the interleukin-1 receptor 1 pathway in breast cancer tumourigenesis and metastasis using a mouse model."AuthorsDagenais M,Dupaul-Chicoine J,Douglas T,Champagne C,Morizot A,Saleh M3. PloS oneEDAG promotes the expansion and survival of human CD34+ cells."17-0452 was used in Flow cytometry/Cell sorting to provide novel insight into EDAG in regulation of expansion and survival of human hematopoietic stem/progenitor cells."AuthorsZhao K,Zheng WW,Dong XM,Yin RH,Gao R,Li X,Liu JF,Zhan YQ,Yu M,Chen H,Ge CH,Ning HM,Yang XM,Li CY4. The Journal of clinical investigationLoss of pleckstrin-2 reverts lethality and vascular occlusions in JAK2V617F-positive myeloproliferative neoplasms."17-0452 was used in Flow cytometry/Cell sorting to identify pleckstrin-2 as an effector of the JAK2/STAT5 pathway and a key factor in the pathogenesis of JAK2V617F-induced myeloproliferative neoplasms."AuthorsZhao B,Mei Y,Cao L,Zhang J,Sumagin R,Yang J,Gao J,Schipma MJ,Wang Y,Thorsheim C,Zhao L,Stalker T,Stein B,Wen QJ,Crispino JD,Abrams CS,Ji P5. PloS oneMultiple functional therapeutic effects of TnP: A small stable synthetic peptide derived from fish venom in a mouse model of multiple sclerosis....By clicking the link above, you will be redirected to the Benchsci website.Disclaimer

技术参数

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