OCT3/4 Monoclonal Antibody (EM92), eFluor 660, eBioscience™

货号： 50-5841-82, 50-5841-80

规格：100 ug,25 ug

价格：4909,2428

产品类型：流式抗体

品牌：eBioscience

抗原：OCT3/4

物种：人/小鼠

宿主：大鼠

抗体亚型：IgG2a, kappa

克隆号：EM92

荧光染料：Purified

类型:	单抗	同型对照：	Rat IgG2a kappa Isotype Control (eBR2a), eFluor 660, eBioscience™
免疫原性：		用法：	1 µg/mL(Flow)

Product Specific InformationDescription: The EM92 monoclonal antibody reacts with mouse and human Oct3/4, encoded by the Pou5F1 gene. Oct3/4 is a POU domain-containing transcription factor that is critical for maintaining embryonic stem (ES) and induced pluripotent stem (iPS) cells in a pluripotent state, and is expressed by ES, embryonic germ cells and embryonic carcinoma cell lines. In cells of the inner cell mass (ICM), reduction of Oct3/4 expression causes dedifferentiation to trophoectoderm, whereas increased expression results in differentiation to mesoderm and primitive endoderm. Oct3/4 regulates the expression of several genes, including FGF-4, UTF1, Sox2, Fbx15, Rex1 and osteopontin through distinct mechanisms. Furthermore, Oct3/4 frequently acts synergistically with Sox2 to regulate target gene expression, as is the case with FGF-4. It has been demonstrated that Oct3/4 expression in ES cells can be negatively regulated by either treatment with retinoic acid, or by removal of leukemia-inhibitory factor (LIF).Applications Reported: This EM92 antibody has been reported for use in intracellular staining followed by flow cytometric analysis.Applications Tested: This EM92 antibody has been tested by intracellular staining and flow cytometric analysis of the F9 cell line using the Foxp3/Transcription Factor Staining Buffer Set (cat. 00-5523) and protocol. This can be used at less than or equal to 1 µg per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test. It is recommended that the antibody be carefully titrated for optimal performance in the assay of interest.eFluor® 660 is a replacement for Alexa Fluor® 647. eFluor® 660 emits at 659 nm and is excited with the red laser (633 nm). Please make sure that your instrument is capable of detecting this fluorochome.Excitation: 633-647 nm; Emission: 668 nm; Laser: Red Laser.Filtration: 0.2 µm post-manufacturing filtered.Target InformationPOU5F1, also commonly known as Oct-4, is a maternally expressed octamer-binding protein that was the first transcription factor described for the early stages of development. The role of POU5F1 in embryonic development suggested that it might be useful in the creation of stem cells that might be useful in cell replacement therapies in the treatment of several degenerative diseases. Artificial stem cells, termed induced pluripotent stem (iPS) cells, can be created by expressing POU5F1 and the transcription factors Sox2, Klf4 and Lin28 along with c-Myc in mouse fibroblasts. More recently, experiments have demonstrated that iPS cells could be generated using expression plasmids expressing POU5F1, Sox2, KlfF4 and c-Myc, eliminating the need for virus introduction, thereby addressing a safety concern for potential use of iPS cells in regenerative medicine.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

数据

OCT3/4 Antibody (50-5841-82) in FlowIntracellular staining of F9 cells with 0.5 µg of Rat IgG2a K Isotype Control eFluor® 660 (Product # 50-4321-82) (blue histogram) or Anti-Human/Mouse OCT3/4 eFluor® 660 (purple histogram) using the Foxp3 Staining Buffer Set (Product # 00-5523-00) and protocol. Total cells were used for analysis.

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参考文献：

1.Nucleic acids researchMulti-kilobase homozygous targeted gene replacement in human induced pluripotent stem cells."50584182 was used in flow cytometry to describe methods to efficiently 'knock-in' multi-kilobase genes in human induced pluripotent stem cells"AuthorsByrne SM,Ortiz L,Mali P,Aach J,Church GM2.Tissue engineering. Part ADevelopmental-like bone regeneration by human embryonic stem cell-derived mesenchymal cells."50-5841 was used in Flow cytometry/Cell sorting to evaluate the in vivo osteogenesis potential of mesenchymal-like cells derived from human embryonic stem cells."AuthorsKuhn LT,Liu Y,Boyd NL,Dennis JE,Jiang X,Xin X,Charles LF,Wang L,Aguila HL,Rowe DW,Lichtler AC,Goldberg AJ3.PloS oneHighly upregulated Lhx2 in the Foxn1-/- nude mouse phenotype reflects a dysregulated and expanded epidermal stem cell niche.

技术参数

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