CD15 Monoclonal Antibody (HI98), PE, eBioscience™
货号：12-0159-41,12-0159-42
规格：25 tests,100 tests
价格：1188,2596
产品类型：流式抗体
品牌：eBioscience
抗原：CD15
物种：人
宿主：小鼠
抗体亚型：IgM
克隆号：HI98
荧光染料：PE
| 抗体类型: | 单抗 | 同型对照： | IgM, kappa |
| 免疫原性： | | 用法： | 5uL（0.125µg）/test(Flow） |
Description: The HI98 monoclonal antibody reacts with human CD15, Lewis X. This 3-fucosyl-N-acetyllactosamine carbohydrate moiety is expressed by granulocytes. Monocytes express this structure at varying degree while lymphocytes are negative.Applications Reported: This HI98 antibody has been reported for use in flow cytometric analysis.Applications Tested: This HI98 antibody has been pre-titrated and tested by flow cytometric analysis of normal human peripheral blood cells. This can be used at 5 µL (0.125 µg) per test. A test is defined as the amount (µg) of antibody that will stain a cell sample in a final volume of 100 µL. Cell number should be determined empirically but can range from 10^5 to 10^8 cells/test.Excitation: 488-561 nm; Emission: 578 nm; Laser: Blue Laser, Green Laser, Yellow-Green Laser.Filtration: 0.2 µm post-manufacturing filtered.Background/Target InformationCD15 (Lewis X, Le(x); stage specific embryonic antigen-1, SSEA-1) is a trisacharide determinant (3-fucosyl-N-acetyllactosamine) expressed on several glycolipids, glycoproteins and proteoglycans of various cell types, e.g. granulocytes, mast cells, monocytes, macrophages, cells of gastric mucosa, nervous system or various tumour cells. There are several variants of Lewis x, such as sialyl-Lewis x or sulphated Lewis x. Cells with high surface expression of Le(x) antigen exhibit strong self-aggregation, based on calcium-dependent Le(x)-Le(x) interaction. This process is involved for example in embryo compaction or in autoaggregation of teratocarcinoma cells. Sialyl-Le(x) and its isomer sialyl-Le(a) are ligands of selectins. CD15 expression has been extensively used to confirm diagnosis of Hodgkin and acutes disease.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.
| 数据 |
CD15 Antibody (12-0159-42) in FlowStaining of normal human peripheral blood cells with Mouse IgM Isotype Control PE (blue histogram) or Anti-Human CD15 PE (purple histogram). Cells in the granulocyte gate were used for analysis. |
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