Goat anti-Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody, Alexa Fluor 350/羊抗鼠IgG (H+L) 交叉吸附荧光二抗,Alexa Fluor 350

货号：A-11045

规格：500 µL

价格：3223

产品类型：荧光二抗

品牌：Thermo Fisher

抗原：Gamma Immunoglobins Heavy and Light chains

物种：小鼠

宿主：山羊

抗体亚型：IgG

荧光染料：Alexa Fluor 350

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：
浓度：	2mg/mL	用法：	1-10µg/mL(ICC);1-10µg/mL（IF); 1-10µg/mL(IHC);1-10µg/mL（Flow)

靶标信息Anti-Mouse secondary antibodies are affinity-purified antibodies with well-characterized specificity for mouse immunoglobulins and are useful in the detection, sorting or purification of its specified target. Secondary antibodies offer increased versatility enabling users to use many detection systems (e.g. HRP, AP, fluorescence). They can also provide greater sensitivity through signal amplification as multiple secondary antibodies can bind to a single primary antibody. Most commonly, secondary antibodies are generated by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (i.e. immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents.

数据

Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11045) in IFMuntjac cells were treated with 10 µM EdU (Product # A10044) for 45 min; cells were then fixed and permeabilized, and EdU that had been incorporated into newly synthesized DNA was detected using the far red-fluorescent Click-iT™ EdU Alexa Fluor® 647 High-Throughput (HCS) Assay (Product # A10208), utilizing the technical tip for converting the HCS assay to conventional fluorescence microscopy. Tubulin was labeled with an anti-α-tubulin antibody (Product # A11126) and visualized with Alexa Fluor® 350 Goat Anti-Mouse IgG (Product # A-11045, A21049). The Golgi complex was stained with green-fluorescent Alexa Fluor® 488 conjugate of lectin HPA from Helix pomatia (edible snail) (Product # L11271), and peroxisomes were labeled with an anti-peroxisome antibody and visualized with orange-fluorescent Alexa Fluor® 555 Donkey Anti-Rabbit IgG (Product # A-31572). Mouse IgG (H+L) Cross-Adsorbed Secondary Antibody (A-11045) in IF Microtubules of fixed bovine pulmonary artery endothelial cells localized with mouse monoclonal anti-a-tubulin antibody (Product # A11126), which was subsequently visualized with Alexa Fluor® 350 goat anti-mouse IgG antibody (Product # A-11045). Next, the F-actin was labeled with Alexa Fluor® 594 phalloidin (Product # A12381). Finally, the cells were incubated with Alexa Fluor® 488 wheat germ agglutinin (Product # W11261) to stain components of endosomal pathways. The superimposed and pseudocolored images were acquired sequentially using bandpass filter sets appropriate for DAPI, the Texas Red® dye and fluorescein, respectively.

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参考文献：

1.Molecular cancer therapeuticsPreclinical Evaluation of UAB30 in Pediatric Renal and Hepatic Malignancies.2.Nature communicationsReversal of axonal growth defects in an extraocular fibrosis model by engineering the kinesin-microtubule interface.3.Journal of molecular and cellular cardiologyα-MHC MitoTimer mouse: In vivo mitochondrial turnover model reveals remarkable mitochondrial heterogeneity in the heart.4.Journal of visualized experiments : JoVEThe analysis of neurovascular remodeling in entorhino-hippocampal organotypic slice cultures.5.NatureBidirectional electromagnetic control of the hypothalamus regulates feeding and metabolism.

技术参数

产品应用 Flow；ICC；IF；IHC

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