Donkey anti-Rabbit IgG (H+L) Highly Cross-Adsorbed Secondary Antibody, Alexa Fluor 350/驴抗兔IgG (H+L) 高交叉吸附 荧光二抗,Alexa Fluor 350

货号：A10039

规格：500 µL

价格：3624

产品类型：荧光二抗

品牌：Thermo Fisher

物种：兔

宿主：驴

抗体亚型：IgG

荧光染料：Alexa Fluor 350

点击查看所有Alexa Fluor 荧光二抗

抗体类型:	荧光二抗	同型对照：	IgG
免疫原性：	Gamma Immunoglobin	用法：	1-10µg/mL(ICC);1-10µg/mL（IF); 1-10µg/mL(IHC);1-10µg/mL（Flow);

These donkey anti-rabbit IgG (H+L) whole secondary antibodies have been affinity-purified and show minimum cross-reactivity to bovine, chicken, goat, guinea pig, hamster, horse, human, mouse, rat, and sheep serum proteins. Cross-adsorption or pre-adsorption is a purification step to increase specificity of the antibody resulting in higher sensitivity and less background staining. The secondary antibody solution is passed through a column matrix containing immobilized serum proteins from potentially cross-reactive species. Only the nonspecific-binding secondary antibodies are captured in the column, and the highly specific secondaries flow through. The benefits of this extra step are apparent in multiplexing/multicolor-staining experiments (e.g., flow cytometry) where there is potential cross-reactivity with other primary antibodies or in tissue/cell fluorescent staining experiments where there may be the presence of endogenous immunoglobulins.Alexa Fluor dyes are among the most trusted fluorescent dyes available today. Invitrogen™ Alexa Fluor 350 dye is a bright, blue-fluorescent dye with excitation ideally suited to the 350 nm laser line. For stable signal generation in imaging and flow cytometry, Alexa Fluor 350 dye is pH-insensitive over a wide molar range. Probes with high fluorescence quantum yield and high photostability allow detection of low-abundance biological structures with great sensitivity. Alexa Fluor 350 dye molecules can be attached to proteins at high molar ratios without significant self-quenching, enabling brighter conjugates and more sensitive detection. The degree of labeling for each conjugate is typically 2-8 fluorophore molecules per IgG molecule; the exact degree of labeling is indicated on the certificate of analysis for each product lot.Using conjugate solutions: Centrifuge the protein conjugate solution briefly in a microcentrifuge before use; add only the supernatant to the experiment. This step will help eliminate any protein aggregates that may have formed during storage, thereby reducing nonspecific background staining. Because staining protocols vary with application, the appropriate dilution of antibody should be determined empirically. For the fluorophore-labeled antibodies a final concentration of 1-10 µg/mL should be satisfactory for most immunohistochemistry and flow cytometry applications.Background/Target InformationWe offer an extensive line of Invitrogen™ secondary antibody conjugates with well-characterized specificity and labeled with a wide selection of premium fluorescent dyes, including Invitrogen™ Alexa Fluor™ fluorescent dyes. Fluorescent secondary antibody conjugates are useful in the detection, sorting, or purification of its specified target and ideal for fluorescence microscopy and confocal laser scanning microscopy, flow cytometry, and fluorescent western detection. The breadth of fluorescent markers we offer allows our reagents to be tailored to almost any fluorescent detection system.Secondary antibodies may be provided in three formats: whole IgG, divalent F(ab')2 fragments, and monovalent Fab fragments. Because of the high degree of conservation in the structure of many immunoglobulin domains, most class-specific secondary antibodies must be affinity-purified and cross-adsorbed to achieve minimal cross-reaction with other immunoglobulins.Our secondary antibody conjugates are most commonly prepared by immunizing the host animal with a pooled population of immunoglobulins from the target species and can be further purified and modified (e.g., immunoaffinity chromatography, antibody fragmentation, label conjugation, etc.) to generate highly specific reagents. In the first round of purification, whole immunoglobulins binding to the immunizing antibody are recovered and mainly consist of the ~150-kDa IgG class. Further purification, for example, with Protein A or G, removes all unwanted immunoglobulin classes except the affinity-purified antibodies that react with the target-specific immunoglobulin heavy and/or light chains.For Research Use Only. Not for use in diagnostic procedures. Not for resale without express authorization.

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参考文献：

1.Methods in molecular biology (Clifton, N.J.)Morphological and Functional Characterization of the Ciliary Pocket by Electron and Fluorescence Microscopy."A10039 was used in immunocytochemistry to review methods to study endocytosis at the ciliary pocket"AuthorsGhossoub R,Lindbæk L,Molla-Herman A,Schmitt A,Christensen ST,Benmerah A2.Scientific reportsHILI destabilizes microtubules by suppressing phosphorylation and Gigaxonin-mediated degradation of TBCB."A10039 was used in immunocytochemistry to report that Human PIWIL2 suppresses microtubule polymerization and promotes cell proliferation, migration and invasion via TBCB"AuthorsTan H,Liao H,Zhao L,Lu Y,Jiang S,Tao D,Liu Y,Ma Y3.The FEBS journalNewly synthesized cAMP is integrated at a membrane protein complex signalosome to ensure receptor response specificity."A10039 was used in immunocytochemistry to demonstrate that signalosomes have their own machinery to synthesize and regulate a sequestered cAMP pool"AuthorsGuinzberg R,Díaz-Cruz A,Acosta-Trujillo C,Vilchis-Landeros MM,Vázquez-Meza H,Lozano-Flores C,Chiquete-Felix N,Varela-Echavarría A,Uribe-Carvajal S,Riveros-Rosas H,Piña E4. Neurochemical researchProtein Interacting C-Kinase 1 Modulates Surface Expression of P2Y6 Purinoreceptor, Actin Polymerization and Phagocytosis in Microglia."A10039 was used in immunocytochemistry to examine the interaction of protein interacting with C-kinase-1 with UDP-activated P2Y6 receptors"AuthorsZhu J,Wang Z,Zhang N,Ma J,Xu SL,Wang Y,Shen Y,Li YH 5. Burns and traumaPartial epithelial-mesenchymal transition in keloid scars: regulation of keloid keratinocyte gene expression by transforming growth factor-β1."A10039 was used in immunohistochemistry to examine the role of epithelial-mesenchymal transition in keloid pathology"AuthorsHahn JM,McFarland KL,Combs KA,Supp DM

技术参数

产品应用 Flow；ICC；IF；IHC

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